Bioactive properties of streptomyces may affect the dominance of Tricholoma matsutake in shiro
Bioactive properties of streptomyces may affect the dominance of Tricholoma matsutake in shiro
复制标题
链霉菌的生物活性特性可能影响松茸在白中的优势
DOI：
10.1007/s13199-020-00678-9
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发表时间：
2020
期刊：
影响因子：
2.5
通讯作者：
Lian Chunlan
中科院分区：
文献类型：
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作者：
Vaario Lu-Min;Asamizu Shumpei;Sarjala Tytti;Matsushita Norihisa;Onaka Hiroyasu;Xia Yan;Kurokochi Hiroyuki;Morinaga Shin-Ichi;Huang Jian;Zhang Shijie;Lian Chunlan
松茸是松茸子实体邻近（shiro）土壤中的优势真菌物种。为了了解松茸优势背后的机制，我们研究了松茸优势白土和非白土中的细菌群落，从白土和白松幼苗中分离出来自松茸菌根根尖的链霉菌菌株。此外，我们调查了三种链霉菌。因其抑制真菌生长和赤松幼苗根伸长的能力，以及两个菌株的抗真菌和抗氧化特性。我们的结果表明，放线菌是白土中最丰富的门。然而，如 PERMANOVA 分析所示，shiro 和非 shiro 土壤之间的放线菌群落组成（门或目水平）差异并不显着。属于放线菌属的链霉菌属存在于松茸菌根上，尽管数量很少。两项抗真菌测定显示，三种链霉菌的肉汤。对不同森林土壤真菌的生长以及幼苗的根系伸长有抑制、中和或促进作用。两种菌株的提取物，其中一种是从 P. densiflorase幼苗，抑制病原菌或外生菌根真菌的生长。效果取决于培养菌株所用的培养基，而不取决于提取所用的溶剂。 2 链霉菌属在三分之一的铁还原抗氧化能力测定中，显示出抗氧化活性。观察到的特性似乎在松茸四郎土壤中具有多种功能，它们可能有助于保护四郎地区。松茸占主导地位。
Tricholoma matsutakeis known to be the dominant fungal species in matsutake fruitbody neighboring (shiro) soil. To understand the mechanisms behind matsutake dominance, we studied the bacterial communities in matsutake dominant shiro soil and non-shiro soil, isolated the strains ofStreptomycesfrom matsutake mycorrhizal root tips both from shiro soil and from thePinus densifloraseedlings cultivated in shiro soil. Further, we investigated threeStreptomycesspp. for their ability to inhibit fungal growth andPinus densifloraseedling root elongation as well as two strains for their antifungal and antioxidative properties.Our results showed that Actinobacteria was the most abundant phylum in shiro soil. However, the differences in the Actinobacterial community composition (phylum or order level) between shiro and non-shiro soils were not significant, as indicated by PERMANOVA analyses. A genus belonging to Actinobacteria,Streptomyces, was present on the matsutake mycorrhizas, although in minority. The two antifungal assays revealed that the broths of threeStreptomycesspp. had either inhibitory, neutral or promoting effects on the growth of different forest soil fungi as well as on the root elongation of the seedlings. The extracts of two strains, including one isolated from theP. densifloraseedlings, inhibited the growth of either pathogenic or ectomycorrhizal fungi. The effect depended on the medium used to cultivate the strains, but not the solvent used for the extraction. TwoStreptomycesspp. showed antioxidant activity in one out of three assays used, in a ferric reducing antioxidant power assay. The observed properties seem to have several functions in matsutake shiro soil and they may contribute to the protection of the shiro area forT. matsutakedominance.