Bacillus fermenti sp. nov., an indigo-reducing obligate alkaliphile isolated from indigo fermentation liquor for dyeing
Bacillus fermenti sp. nov., an indigo-reducing obligate alkaliphile isolated from indigo fermentation liquor for dyeing
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DOI：
10.1099/ijsem.0.002636
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发表时间：
2018-04-01
影响因子：
2.8
通讯作者：
Yumoto, Isao
中科院分区：
文献类型：
--
作者：
Hirota, Kikue;Nishita, Masatoshi;Yumoto, Isao
从以苏库莫[Polygonum靛蓝（Polygonum tinctorium Lour.）叶]作为基本成分，并从日本北海道伊达市的工艺中心获得。16 S rRNA基因序列分析表明，与Bf-1（T）菌株最接近的是海洋芽孢杆菌DSM 100413（T）（16 S rRNA基因序列相似性为98.3%）、波斯芽孢杆菌DSM 25386（T）（98.2%）和Rigiliprofundi芽孢杆菌LMG 28275（T）（97.7%）。菌株Bf-1（T）的16 SrRNA基因序列与菌株Bf-2和Bf-4的16 SrRNA基因序列几乎一致（99.9%）。菌株Bf-1（T）的细胞染色为革兰氏阳性，并形成通过一对亚极鞭毛实现运动的直杆。菌株Bf-1（T）在15 - 45 ℃的温度下生长，最佳生长温度为33-40 ℃。该菌株在pH 8-12范围内生长，最适生长pH为10。检测到的类异戊二烯醌为甲基萘醌-7（MK-7），DNA G+ C含量为41.7%。全细胞脂肪酸谱主要（> 10%）由iso-C-15：0和iso-C-16：0组成。系统发育相关的邻居，虽然显示出与菌株Bf-1（T）的高16 S rRNA基因序列相似性（> 97.6%），但在DNA-DNA杂交实验中显示出小于9%的相关性。根据这项多相研究的证据，这些分离株代表了一个新的物种，并建议将其命名为发酵芽孢杆菌。该种的模式菌株为Bf-1（T）（= JCM 31807（T）= NCIMB 15079（T））。
The indigo-reducing, facultatively anaerobic and obligately alkaliphilic strains Bf-1(T), Bf-2 and Bf-4 were isolated from an indigo fermentation liquor used for dyeing, which uses sukumo [composted Polygonum indigo (Polygonum tinctorium Lour.) leaves] as a basic ingredient and was obtained from a craft centre in Date City, Hokkaido, Japan. The 16S rRNA gene sequence analyses indicated that the closest neighbours of strain Bf-1(T) are Bacillus maritimus DSM 100413(T) (98.3% 16S rRNA gene sequence similarity), Bacillus persicus DSM 25386(T) (98.2 %) and Bacillus rigiliprofundi LMG 28275(T) (97.7 %). The 16S rRNA gene sequence of strain Bf-1(T) was almost identical to the sequences of strains Bf-2 and Bf-4 (99.9 %). Cells of strain Bf-1(T) stained Gram-positive and formed straight rods that achieved motility through a pair of subpolar flagella. Strain Bf-1(T) grew at temperatures of between 15 and 45 degrees C with optimum growth at 33-40 degrees C. The strain grew in the pH range of pH 8-12, with optimum growth at pH 10. The isoprenoid quinone detected was menaquinone-7 (MK-7), and the DNA G+ C content was 41.7 %. The whole-cell fatty acid profile mainly (> 10 %) consisted of iso-C-15 : 0 and iso-C-16 : 0. Phylogenetically related neighbours, although demonstrating high 16S rRNA gene sequence similarity (> 97.6 %) with strain Bf-1(T), exhibited less than 9% relatedness in DNA- DNA hybridization experiments. Based on evidence from this polyphasic study, the isolates represent a novel species, for which the name Bacillus fermenti sp. nov. is proposed. The type strain of this species is Bf-1(T) (= JCM 31807(T) = NCIMB 15079(T)).