IMMUNOHISTOCHEMICAL LOCALIZATION OF PROTEOGLYCANS IN HUMAN PERIODONTIUM
IMMUNOHISTOCHEMICAL LOCALIZATION OF PROTEOGLYCANS IN HUMAN PERIODONTIUM
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DOI：
10.1177/41.11.8409375
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发表时间：
1993-11-01
影响因子：
3.2
通讯作者：
LARJAVA, H
中科院分区：
文献类型：
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作者：
HAKKINEN, L;OKSALA, O;LARJAVA, H
蛋白聚糖（PG）是细胞外和细胞表面相关的大分子，其调节细胞粘附、细胞生长、基质形成和结合生长因子。在这项工作中，我们研究了核心蛋白的四个PG（核心蛋白聚糖，biglycan，一个大分子量的PG，和CD44）在人类牙龈和牙周膜的冷冻组织切片的免疫组织化学染色的特异性抗体的分布。核心蛋白聚糖是该组织的主要PG，定位于牙龈和牙周结缔组织中的胶原纤维束上。核心蛋白聚糖的染色在上皮下区域最强烈。双糖链蛋白聚糖是人牙周组织的次要PG组分，在口腔上皮下的结缔组织中显示出一定程度的积累。在免疫组化水平，双糖蛋白聚糖似乎形成细胞外基质纤维上的精细的免疫球蛋白样结构。大分子量PG的定位不同于核心蛋白聚糖和双糖链蛋白聚糖。它集中在牙龈和牙周膜的深层结缔组织区域，仅在上皮下区域有微弱的表达。CD44主要集中在口腔上皮基底层和棘层的细胞接触区。在牙龈和牙周膜的结缔组织中，CD44定位于成纤维细胞表面。连接上皮下的结缔组织区含有相对少量的PG。结果表明，人类牙周组织的不同部位含有典型的各种PG，这表明每种PG在其积聚的位置具有特定的功能。
Proteoglycans (PGs) are extracellular and cell surface-associated macromolecules that regulate cell adhesion, cell growth, matrix formation, and bind growth factors. In this work we studied the distribution of core proteins of four PGs (decorin, biglycan, a large molecular weight PG, and CD44) in human gingiva and periodontal ligament by immunohistochemical staining of frozen tissue sections with specific antibodies. Decorin, a major PG of this tissue, was localized on collagen fiber bundles in the gingival and periodontal connective tissues. Staining for decorin was most intense at the subepithelial region. Biglycan was a minor PG component of the human periodontium, showing some accumulation in connective tissue under the oral epithelium. At the immunohistochemical level, biglycan appeared to form fine filament-like structures on extracellular matrix fibers. Localization of large molecular weight PG differed from that of decorin and biglycan. It was concentrated in deep connective tissue areas of the gingiva and in the periodontal ligament, and was only weakly present at the subepithelial region. CD44 was mainly concentrated in cell-cell contact areas of basal and spinous layers of oral epithelium. In the connective tissue of gingiva and periodontal ligament, CD44 was localized on fibroblast cell surfaces. Connective tissue area under the junctional epithelium contained relatively small amounts of PGs. The results indicate that different parts of human periodontium contain a typical variety of PGs, suggesting a specific function for each PG species in the location at which they accumulate.