Mycoplasma pneumoniae: a reduced-genome intracellular bacterial pathogen
Mycoplasma pneumoniae: a reduced-genome intracellular bacterial pathogen
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DOI：
10.1016/s1567-1348(02)00151-x
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发表时间：
2003-05-01
影响因子：
3.2
通讯作者：
Baquero, Fernando
中科院分区：
文献类型：
--
作者：
Meseguer, Maria A.;Alvarez, Alberto;Baquero, Fernando
肺炎支原体通常被认为是一种细胞外（或膜相关）微生物。然而，最近阐明的这种病原体的基因组结构强烈表明，这种生物体可能已经受到还原性遗传进化的过程，这是细胞内细菌的特征。我们研究了肺炎支原体RYC 15989菌株，从非典型肺炎和急性心包炎患者的心包活检样本中回收。研究了该菌株与人肝细胞Hep-G2和小鼠神经母细胞瘤N2-A细胞系的相互作用。共聚焦激光扫描显微镜和电子显微镜证据的荧光染料标记的肺炎支原体在细胞系感染的有机体在体外的细胞内的位置。这一发现为肺炎支原体的细胞侵袭能力提供了初步证据，并为肺炎支原体在宿主感染中的致病潜力提供了新的认识。(C)2002 Elsevier Science B. V.保留所有权利。
Mycoplasma pneumoniae has classically been considered an extracellular (or membrane-associated) organism. Nevertheless, the recently elucidated genomic structure of this pathogen strongly suggest that this organism may have been subjected to the process of reductive genetic evolution which is characteristic of intracellular bacteria. We studied the Mycoplasma pneumoniae RYC15989 strain, recovered from a pericardial biopsy sample from a patient with atypical pneumonia and acute pericarditis. The interaction of this strain with human hepatocytes Hep-G2 and mouse neuroblastoma N2-A cell lines was investigated. Confocal laser scanning microscopy and electronic microscopy evidence is presented of the intracellular location of fluorochrome-labelled Mycoplasma pneumoniae in cell lines infected with the organism in vitro. This finding provides preliminary evidence of cellular invasive capacity of Mycoplasma pneumoniae and casts some new light on the pathogenic potential of Mycoplasma pneumoniae in host infection. (C) 2002 Elsevier Science B.V. All rights reserved.