Measuring the microelastic properties of biological material.
Measuring the microelastic properties of biological material.
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DOI：
10.1016/s0006-3495(92)81692-2
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发表时间：
1992-10
影响因子：
3.4
通讯作者：
N. Tao;S. Lindsay;S. Lees
中科院分区：
文献类型：
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作者：
N. Tao;S. Lindsay;S. Lees
我们用原子力显微镜(AFM)测量了水合奶牛胫骨表面各点的局部刚性系数。这些数据要么是通过改变接触力时发生的对比度变化获得的，要么是通过在固定点获得的力-距离曲线获得的。这两种方法产生相同的刚性模量值(在给定点)。在低分辨率下，弹性形态和地形反映了光学和电子显微照片中的特征。在高分辨率下，我们看到弹性特性在小到50纳米的距离上发生了戏剧性的变化。
We have used the atomic force microscope (AFM) to measure the local rigidity modulus at points on the surface of a section of hydrated cow tibia. These data are obtained either from contrast changes that occur as the contact force is altered, or from force versus distance curves obtained at fixed points. These two methods yield the same values for rigidity modulus (at a given point). At low resolution, the elastic morphology and topography mirror the features seen in optical and electron micrographs. At high resolution we see dramatic variations in elastic properties across distances as small as 50 nm.