Galectin-4, a novel predictor for lymph node metastasis in lung adenocarcinoma.
Galectin-4, a novel predictor for lymph node metastasis in lung adenocarcinoma.
复制标题
DOI：
10.1371/journal.pone.0081883
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Yao T
中科院分区：
文献类型：
--
作者：
Hayashi T;Saito T;Fujimura T;Hara K;Takamochi K;Mitani K;Mineki R;Kazuno S;Oh S;Ueno T;Suzuki K;Yao T
转移仍然是癌症中的一个主要问题，发现预测转移能力的生物标志物对于开发更好的治疗肺腺癌的治疗策略至关重要。通过使用蛋白质组学的方法，我们的目的是确定新的预测肺腺癌淋巴结转移。双向十二烷基硫酸钠聚丙烯酰胺凝胶电泳显示6个点的差异表达的淋巴结转移阳性和淋巴结转移阴性组的发现集。随后的质谱分析显示，其中2个斑点源自半乳凝素-4，蛋白质印迹分析证实了转移性样本中半乳凝素-4的过表达。半乳糖凝集素-4的预测价值通过免疫组化分析确认，该分析包括707例手术切除的肺腺癌标本（I期至IV期）。我们观察到148例肺腺癌（20.9%）表达半乳糖凝集素-4，其与疾病进展的变量如肿瘤大小（p<0.0001）、胸膜浸润（p =0.0071）、静脉浸润（p= 0.0178）、淋巴结状态（p = 0.0007）和TNM分期（p<0.0001）显著相关。 多因素分析显示，Galectin-4表达是淋巴结转移的独立预测因子之一，同时伴有实性为主和微乳头状组织学类型。此外，半乳糖凝集素-4的表达被认为是一个独立的预测淋巴结转移和一个不利的生存因素，在肺腺癌的腺泡占主导地位的类型。Galectin-4在肺腺癌的转移过程中起重要作用。半乳凝素-4表达的免疫组织化学检测与特定组织学检测一起可能有助于预测肺腺癌的转移潜力。
Metastasis is still a major issue in cancer, and the discovery of biomarkers predicting metastatic capacity is essential for the development of better therapeutic strategies for treating lung adenocarcinoma. By using a proteomic approach, we aimed to identify novel predictors for lymph node metastasis in lung adenocarcinoma. Two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis showed 6 spots differentially expressed between lymph node metastasis-positive and lymph node metastasis-negative groups in a discovery set. Subsequent mass spectrometry showed that 2 of these spots were derived from galectin-4, and western blot analysis confirmed the overexpression of galectin-4 in metastatic samples. The predictive value of galectin-4 was confirmed by immunohistochemical analysis for a validation set consisting of 707 surgically resected specimens of lung adenocarcinomas (stages I to IV). We observed that 148 lung adenocarcinomas (20.9%) expressed galectin-4, which was significantly associated with variables of disease progression such as tumor size (p<0.0001), pleural invasion (p = 0.0071), venous invasion (p = 0.0178), nodal status (p = 0.0007), and TNM stage (p<0.0001). By the multivariate analysis, Galectin-4 expression was revealed as one of the independent predictor for lymph node metastasis, together with solid predominant and micropapillary histologic pattern. Furthermore, galectin-4 expression was revealed to be an independent predictor for lymph node metastasis and an adverse survival factor in patients with lung adenocarcinoma of acinar predominant type. Galectin-4 plays an important role in metastatic process of lung adenocarcinoma. Immunohistochemical testing for galectin-4 expression may be useful together with the detection of specific histology to predict the metastatic potential of lung adenocarcinoma.
登录
查看更多内容
影响因子：
158.5
作者：
Lynch, TJ;Bell, DW;Haber, DA
通讯作者：
Haber, DA
影响因子：
14.8
作者：
Horie, Akira;Tomita, Takeo;Nishiyama, Makoto
通讯作者：
Nishiyama, Makoto
影响因子：
3
作者：
Huflejt, ME;Leffler, H
通讯作者：
Leffler, H
影响因子：
11.2
作者：
Jan, Yi-Hua;Tsai, Hong-Yuan;Hsiao, Michael
通讯作者：
Hsiao, Michael
影响因子：
6.4
作者：
Saito, Seiichi;Murayama, Yasuko;Arai, Yoichi
通讯作者：
Arai, Yoichi