A set of highly informative rat simple sequence length polymorphism (SSLP) markers and genetically defined rat strains
A set of highly informative rat simple sequence length polymorphism (SSLP) markers and genetically defined rat strains
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DOI：
10.1186/1471-2156-7-19
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发表时间：
2006-04-04
期刊：
影响因子：
2.9
通讯作者：
Serikawa, Tadao
中科院分区：
文献类型：
--
作者：
Mashimo, Tomoji;Voigt, Birger;Serikawa, Tadao
背景：日本国家大鼠生物资源计划致力于收集、保存和分布各种大鼠品系，包括自发突变品系、转基因品系、同基因品系和重组近交系品系。为了评估它们作为人类疾病模型的价值，我们正在使用109个表型参数来表征它们，如临床测量、内部解剖、代谢参数和行为测试，这是大鼠表型组计划的一部分。本文对357个SSLP标记和122个大鼠品系进行了基因分型。结果：根据SSLP标记在大鼠全基因组中的分布模式，筛选出平均间距为7.59Mb的SSLP标记。所有可能的菌株对之间的平均信息标记数为259个，占357个标记的72.5%，显示出高度的多态。结论：这些高信息量的SSLP标记以及在遗传和表型上定义的大鼠品系对于数量性状座位(QTL)分析的实验设计和新遗传资源开发策略的选择是有用的。这些数据和资源可在NBRP-RAT网站上免费获得[1]。
Background: The National Bio Resource Project for the Rat in Japan (NBRP-Rat) is focusing on collecting, preserving and distributing various rat strains, including spontaneous mutant, transgenic, congenic, and recombinant inbred (RI) strains. To evaluate their value as models of human diseases, we are characterizing them using 109 phenotypic parameters, such as clinical measurements, internal anatomy, metabolic parameters, and behavioral tests, as part of the Rat Phenome Project. Here, we report on a set of 357 simple sequence length polymorphism (SSLP) markers and 122 rat strains, which were genotyped by the marker set.Results: The SSLP markers were selected according to their distribution patterns throughout the whole rat genome with an average spacing of 7.59 Mb. The average number of informative markers between all possible pairs of strains was 259 (72.5% of 357 markers), showing their high degree of polymorphism. From the genetic profile of these rat inbred strains, we constructed a rat family tree to clarify their genetic background.Conclusion: These highly informative SSLP markers as well as genetically and phenotypically defined rat strains are useful for designing experiments for quantitative trait loci (QTL) analysis and to choose strategies for developing new genetic resources. The data and resources are freely available at the NBRP-Rat web site [1].