ROLES OF VARIOUS FRACTIONS OF PSEUDOMONAS AERUGINOSA IN ITS PATHOGENESIS .3. IDENTITY OF LETHAL TOXINS PRODUCED IN VITRO AND IN VIVO
ROLES OF VARIOUS FRACTIONS OF PSEUDOMONAS AERUGINOSA IN ITS PATHOGENESIS .3. IDENTITY OF LETHAL TOXINS PRODUCED IN VITRO AND IN VIVO
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DOI：
10.1093/infdis/116.4.481
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发表时间：
1966-01-01
影响因子：
6.4
通讯作者：
LIU, PV
中科院分区：
文献类型：
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作者：
LIU, PV
铜绿假单胞菌在体内产生一种致命毒素，其本质上可能是蛋白质。它不能与任何体外活性相关。用这种毒素免疫动物导致产生一种类型特异性抗体，这种抗体可以中和抗原的毒性作用。然而，这种抗体并不能保护动物免受感染。只有在琼脂表面生长或在肉汤培养液中大力充气时，用普通实验室培养基在体外才能产生类似的致死毒素。这种毒素可以用正戊醇和氯仿的混合物从活细胞中提取出来。该毒素可能与生物体的体细胞抗原无关，其产生不涉及自溶。涉及大面积皮肤的假单胞菌感染的致死效果可能是由于在皮肤中产生的致命毒素的吸收，而不是由于所产生的菌血症的影响。
Pseudomonas aeruginosa produces a lethal toxin in vivo which may be protein in nature. It could not be correlated with any in vitro activity. Immunization of animals with this toxin resulted in the production of a type-specific antibody that neutralized the toxic effects of the antigen. However, the antibody did not protect animals from infection. A similar lethal toxin could be produced in vitro with ordinary laboratory media only when the organism was grown on the surface of agar or the broth culture was vigorously aerated. The toxin could be extracted from live cells with a mixture of n-amyl alcohol and chloroform. The toxin is probably not related to the somatic antigen of the organism and its production does not involve autolysis. The lethal effect of Pseudomonas infections involving a large area of skin was probably due to absorption of the lethal toxin produced in the skin and not due to the effects of the resultant bacteremia.