Potential roles of insect Tropomyosin1-X1 isoform in the process of Candidatus Liberibacter asiaticus infection of Diaphorina citri
Potential roles of insect Tropomyosin1-X1 isoform in the process of Candidatus Liberibacter asiaticus infection of Diaphorina citri
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昆虫原肌球蛋白1-X1亚型在柑橘木虱感染Candidatus Liberibacter asiaticus过程中的潜在作用
DOI：
10.1016/j.jinsphys.2019.02.012
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发表时间：
2019-04-01
影响因子：
2.2
通讯作者：
Yang, Chao
中科院分区：
文献类型：
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作者：
Lu, Zhan-jun;Zhou, Cheng-hua;Yang, Chao
柑橘木虱（Diaphorina citri Kuwayama）是柑橘黄龙病（HLB）的传播媒介。近年来，HLB的控制已通过减少媒介种群来实现。在本研究中，我们鉴定了D. Citri原肌球蛋白（本文称为DcTm 1-X1）。与未感染的ACP相比，CLas感染的ACP中DcTm 1-X1下调。生物信息学分析表明，DcTm 1-X1基因全长2955 bp，编码284个氨基酸，推测分子量为32.15 kDa。系统进化树分析表明，DcTm 1-X1与豌豆蚜的同源物具有较高的氨基酸同源性。RT-qPCR结果显示，DcTm 1-X1在木虱足中的表达量较高。Blue Native PAGE分析和质谱分析表明，DcTm 1-X1与柠檬酸合成酶（CS）和V型质子ATP酶亚基B样（VAT）相互作用。此外，通过RNA干扰（RNAi）敲低DcTm 1-X1显著增加了不同时间点的疟原虫死亡率和CLas感染率。综上所述，我们的研究结果表明DcTm 1-X1可能在CLas应答中发挥重要作用，同时也为进一步研究DcTm 1-X1的功能奠定了基础。
The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama, is the transmitting vector of Candidatus Liberibacter asiaticus (CLas), which causes citrus disease Huanglongbing (HLB). In recent years, control of HLB has been achieved by reducing the vector population. In the present study, we identified an isoform of D. citri tropomyosin (herein designated as DcTm1-X1). DcTm1-X1 was down-regulated in CLas-infected ACPs compared with uninfected ACPs. Bioinformatics analysis revealed that the full-length DcTm1-X1 is 2955 bp and encodes a protein of 284 amino acids with a deduced molecular weight of 32.15 kDa. Phylogenetic tree analysis suggested that DcTm1-X1 shares a high amino acid identity with its homolog in Acyrthosiphon pisum. Higher DcTm1-X1 expression levels were found in the leg of the psyllid by reverse transcription quantitative PCR (RT-qPCR). According to Blue Native PAGE analysis and mass spectrometric analysis, DcTm1-X1 interacts with citrate synthase (CS) and V-type proton ATPase subunit B-like (VAT). In addition, knockdown of DcTm1-X1 by RNA interference (RNAi) significantly increased the mortality rate of nymphs and the infection rate of CLas at different time points. Taken together, our results show that DcTm1-X1 might play an important role in response to CLas, but also lay a foundation for further research on the functions of DcTm1-X1.