Vibrio parahaemolyticus serovar O3:K6 gastroenteritis in northeast Brazil
Vibrio parahaemolyticus serovar O3:K6 gastroenteritis in northeast Brazil
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DOI：
10.1111/j.1365-2672.2008.03782.x
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发表时间：
2008-09-01
影响因子：
4
通讯作者：
Hofer, E.
中科院分区：
文献类型：
--
作者：
Leal, N. C.;da Silva, S. C.;Hofer, E.
目的：为了研究毒力因子和遗传关系的O3血清群的副溶血性弧菌在腹泻爆发的巴西东北部regionsofBrazil.Methods和结果：18个样本的O3：K6和O3：KUT血清型的副溶血性弧菌进行了分析，多重聚合酶链反应（m-PCR）检测的TL，tdh和trh基因，随机扩增多态性DNA（RAPD）使用两个引物，并通过扩增的rDNA 16 S-23 S区域。tdh基因在16份样品中均扩增出，trh基因在16份样品中均未扩增出; rDNA 16 S-23 S的扩增仅产生一个谱型;每个RAPD引物产生两种扩增模式，可将两株tdh（-）神奈川阴性的分离株分组。由于分离株的遗传一致性，用目前的分子技术来阐明暴发或追踪污染源似乎是无用的。从两次暴发和几个孤立的胃肠炎病例中检测到具有大流行性克隆毒力潜力的副溶血性弧菌，指出有必要纳入这种微生物学方法。在巴西的日常监测中，这些细菌被用于阐明其病因。
Aims: To examine the virulence factors and the genetic relationship isolates of the serogroup O3 of Vibrio parahaemolyticus in outbreaks of diarrhoea in the northeast region of Brazil.Methods and Results: Eighteen samples of the O3:K6 and O3:KUT serotypes of V. parahaemolyticus were analysed by multiplex polymerase chain reaction (m-PCR) for detection of the tl, tdh and trh genes, by random-amplified polymorphic DNA (RAPD) using two primers, and by amplification of the rDNA 16S-23S region. The gene tl was amplified in all the samples, tdh in 16 while trh in none; amplification of rDNA 16S-23S generated only one profile; each RAPD primer produced two amplification patterns allowing grouping two tdh(-) Kanagawa-negative isolates.Conclusions: V. parahaemolyticus with characteristics of the pandemic clone appears to be widely disseminated in the studied region. Because of the genetic uniformity of the isolates, elucidation of outbreaks or tracking the source of contamination by the present molecular techniques seems useless.Significance and Impact of the Study: Detection of V. parahaemolyticus with virulence potential of pandemic clone from two outbreaks and from several isolated gastroenteritis cases points out the need for inclusion of this micro-organism in the Brazilian routine monitoring of the diarrhoeas for elucidation of their aetiology.