MicroRNA-143 regulates adipocyte differentiation
MicroRNA-143 regulates adipocyte differentiation
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DOI：
10.1074/jbc.c400438200
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发表时间：
2004-12-10
影响因子：
4.8
通讯作者：
Griffey, R
中科院分区：
文献类型：
--
作者：
Esau, C;Kang, XL;Griffey, R
MicroRNAs （miRNAs）是内源性表达20-24个核苷酸的rna，被认为通过与靶mRNA结合来抑制蛋白质翻译（1-3）。在超过250种预测的人类mirna中，只有少数被赋予了任何生物学功能。为了揭示在脂肪细胞分化过程中重要的miRNAs，我们将86种靶向人miRNAs的反义寡核苷酸（ASOs）转染到培养的人前脂肪细胞中，并评估其调节脂肪细胞分化的能力。通过miRNA微阵列检测了254个miRNA在分化脂肪细胞中的表达。在这里，我们报告了表达数据和功能分析结果的结合，确定了miR-143在脂肪细胞分化中的作用。miR-143水平在脂肪细胞分化过程中升高，抑制miR-143可有效抑制脂肪细胞分化。此外，在aso处理的脂肪细胞中，提出的miR-143靶点ERK5(4)的蛋白水平更高。这些结果表明miR-143参与脂肪细胞分化，并可能通过靶基因ERK5起作用。
MicroRNAs (miRNAs) are endogenously expressed 20-24 nucleotide RNAs thought to repress protein translation through binding to a target mRNA (1-3). Only a few of the more than 250 predicted human miRNAs have been assigned any biological function. In an effort to uncover miRNAs important during adipocyte differentiation, antisense oligonucleotides (ASOs) targeting 86 human miRNAs were transfected into cultured human pre-adipocytes, and their ability to modulate adipocyte differentiation was evaluated. Expression of 254 miRNAs in differentiating adipocytes was also examined on a miRNA microarray. Here we report that the combination of expression data and functional assay results identified a role for miR-143 in adipocyte differentiation. miR-143 levels increased in differentiating adipocytes, and inhibition of miR-143 effectively inhibited adipocyte differentiation. In addition, protein levels of the proposed miR-143 target ERK5 (4) were higher in ASO-treated adipocytes. These results demonstrate that miR-143 is involved in adipocyte differentiation and may act through target gene ERK5.