Neuroprotective effects of NSTyr on cognitive function and neuronal plasticity in rats of chronic cerebral hypoperfusion
Neuroprotective effects of NSTyr on cognitive function and neuronal plasticity in rats of chronic cerebral hypoperfusion
复制标题
NSTyr对慢性脑低灌注大鼠认知功能和神经元可塑性的神经保护作用
DOI：
10.1016/j.brainres.2010.02.037
复制
发表时间：
2010-04-14
期刊：
影响因子：
2.9
通讯作者：
Lu, Yang
中科院分区：
文献类型：
--
作者：
Lin, Qi;Hai, Jian;Lu, Yang
本文研究了N-硬脂酰-L-酪氨酸（NSTyr）对慢性脑低灌注（CCH）大鼠认知功能和神经元可塑性的保护作用。诱导CCH后，NSTyr每天腹腔内给药3个月。采用Morris水迷宫和海马长时程增强（LTP）检测大鼠的认知功能。光镜及Fluoro-Jade B染色观察神经病理改变。采用免疫组织化学和蛋白质印迹法检测海马区MAP-2、GAP-43和突触素的表达，评价神经元的可塑性。与假手术组相比，模型组大鼠的空间记忆功能明显受损，LTP明显降低，海马CA 1区神经元变性。在用NSTyr治疗的模型大鼠中，认知功能得到改善。模型组大鼠海马区MAP-2和突触素蛋白的表达水平低于假手术组，模型组大鼠给予NSTyr后，MAP-2和突触素蛋白的表达水平升高。GAP-43在假手术组、模型组和NSTyr组中的表达无统计学意义。提示NSTyr对CCH后大鼠认知功能有保护作用，其机制可能与海马区神经元变性和可塑性改变有关。(C)2010爱思唯尔有限公司版权所有。
The neuroprotective effects of N-stearoyl-L-tyrosine (NSTyr) on cognitive function and neuronal plasticity during chronic cerebral hypoperfusion (CCH) in rats were investigated. After induction of CCH, NSTyr was administered daily for 3 months intraperitoneally. Cognitive functions were evaluated by Morris water maze and hippocampal long-term potentiation (LTP). Neuropathological changes were examined using light micrograph and Fluoro-Jade B staining. Neuronal plasticity was assessed by measuring the expression of MAP-2, GAP-43 and synaptophysin on hippocampal regions of rats with immunohistochemistry and western blotting. CCH resulted in significant spatial memory impairment and inhibition of LTP, and led to neurodegeneration in the CA1 region of the hippocampus in the model rats compared with the sham-operated rats. In the model rats treated with NSTyr, cognitive function improved. The expression levels of MAP-2 and synaptophysin protein in hippocampal areas in the model rats were less than those in the sham-operated rats, and increased in the model rats treated with NSTyr. However, no statistical significance of GAP-43 expression among the sham, model and NSTyr groups was observed. These data indicate that NSTyr exerts protective effects on cognitive function of rats after CCH, which may be related to the changes of neurodegeneration and neuronal plasticity in the hippocampal area of rats. (C) 2010 Elsevier B.V. All rights reserved.