A high-efficient and stable artificial superoxide dismutase based on functionalized melanin nanoparticles from cuttlefish ink for food preservation
A high-efficient and stable artificial superoxide dismutase based on functionalized melanin nanoparticles from cuttlefish ink for food preservation
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基于墨鱼墨汁功能化黑色素纳米颗粒的高效稳定人工超氧化物歧化酶用于食品保鲜
DOI：
10.1016/j.foodres.2022.112211
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发表时间：
2023-01-01
影响因子：
8.1
通讯作者：
Zhang, Wentao
中科院分区：
文献类型：
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作者：
Liang, Yanmin;Han, Yaru;Zhang, Wentao
天然超氧化物歧化酶（SOD）是由蛋白质和金属辅因子组成，具有良好的抗氧化活性，被广泛应用于食品防腐。但由于SOD酶的稳定性差，在移入膜中的过程中其活性降低，导致应用受到限制。基于天然酶活性中心的结构，采用Cu 2+对墨鱼墨汁中的黑色素纳米颗粒（NMPs）进行功能化修饰，构建了一种高稳定性、高活性、强自由基清除能力的SOD样纳米酶（Cu-NMPs）。为了将构建的模拟酶应用于食品保鲜，将模拟酶包埋于卡拉胶（卡尔）膜中，制备复合膜用于食品包装。结果表明，当Cu-NMPs浓度为10 kig/mL时，中心点O-2（-）率可达80%以上，活性超过60 U/mL天然SOD。另外，樱桃番茄的保鲜试验表明，卡尔/Cu-NMPs复合膜可使樱桃番茄的贮藏时间延长3 d以上。因此，本工作表明，具有先进的催化能力的纳米酶可以构建由金属离子和NMPs，从而成功地结合到食品包装食品保鲜。
Natural superoxide dismutase (SOD), consisting of proteins and metal cofactors, is widely used in food preservation because of its good antioxidant activity. However, due to the poor stability of SOD enzyme, its activity was reduced in the process of moving into the film, resulting in limited application. Based on the structure of the active site of the natural enzyme, Cu2+ was used to functionalize the melanin nanoparticles (NMPs) in ink of cuttlefish, and an SOD-like nanozyme (Cu-NMPs) with high stability, high activity and strong free radical scavenging capacity was constructed. In order to apply the constructed simulated enzyme to food preservation, the simulated enzyme was embedded into carrageenan (Carr) films to prepare the composite film for food packaging. The results showed that when the concentration of Cu-NMPs was 10 kig/mL, the center dot O-2(-) rate could reach more than 80 %, the activity exceeded that of 60 U/mL natural SOD. In addition, the fresh-keeping test of cherry tomatoes showed that Carr/Cu-NMPs composite film extended the storage time of cherry tomatoes by more 3 days. Therefore, the present work showed that nanozymes with advanced catalytic capabilities can be constructed by metal ions and NMPs, thus successfully combined with food packaging for food preservation.