Stu2p: A microtubule-binding protein that is an essential component of the yeast spindle pole body.
Stu2p: A microtubule-binding protein that is an essential component of the yeast spindle pole body.
复制标题
DOI：
10.1083/jcb.139.5.1271
复制
发表时间：
1997-12-01
期刊：
影响因子：
--
通讯作者：
Huffaker TC
中科院分区：
文献类型：
--
作者：
Wang PJ;Huffaker TC
先前我们分离了tub 2 -423，这是酿酒酵母基因编码β-微管蛋白的冷敏感等位基因，其赋予有丝分裂纺锤体功能的缺陷。为了鉴定对纺锤体功能重要的其他蛋白质，我们筛选了tub 2 -423冷敏感性的抑制因子，并获得了一个新基因STU 2的两个等位基因。STU 2是一种必需基因，编码一种蛋白质，其序列与多种生物体中鉴定的蛋白质相似。Stu 2 p主要定位于纺锤体极体（SPB），并在较小程度上沿着纺锤体微管。定位到SPB不依赖于微管的存在，表明Stu 2 p是SPB的组成部分。Stu 2 p还在体外结合微管。我们已经将Stu 2 p的微管结合结构域定位到高度碱性的100个氨基酸区域。该区域包含两个不完美的重复序列，这两个重复序列似乎有助于微管结合到类似的程度。这些结果表明，Stu 2 p可能在SPB的微管末端的附着、组织和/或动力学中发挥作用。
Previously we isolated tub2-423, a cold-sensitive allele of the Saccharomyces cerevisiae gene encoding β-tubulin that confers a defect in mitotic spindle function. In an attempt to identify additional proteins that are important for spindle function, we screened for suppressors of the cold sensitivity of tub2-423 and obtained two alleles of a novel gene, STU2. STU2 is an essential gene and encodes a protein whose sequence is similar to proteins identified in a variety of organisms. Stu2p localizes primarily to the spindle pole body (SPB) and to a lesser extent along spindle microtubules. Localization to the SPB is not dependent on the presence of microtubules, indicating that Stu2p is an integral component of the SPB. Stu2p also binds microtubules in vitro. We have localized the microtubule-binding domain of Stu2p to a highly basic 100-amino acid region. This region contains two imperfect repeats; both repeats appear to contribute to microtubule binding to similar extents. These results suggest that Stu2p may play a role in the attachment, organization, and/or dynamics of microtubule ends at the SPB.