Expression and characteristic of synthetic human epidermal growth factor (hEGF) in transgenic tobacco plants
Expression and characteristic of synthetic human epidermal growth factor (hEGF) in transgenic tobacco plants
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DOI：
10.1007/s10529-007-9438-y
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发表时间：
2007-12-01
影响因子：
2.7
通讯作者：
Shi, Yan-Sheng
中科院分区：
文献类型：
--
作者：
Bai, Jie-Ying;Zeng, Lin;Shi, Yan-Sheng
为提高重组人表皮生长因子（hEGF）在转基因烟草中的积累，构建了重组人表皮生长因子（hEGF）在转基因烟草中的高效表达载体。转基因烟草中hEGF含量可达可溶性蛋白总量的0.3%。通过Vero E6细胞扩增实验和MTT细胞增殖法，转基因烟草生产的hEGF显著刺激Vero E6细胞扩增和增殖，与商业hEGF产品相同。
To improve the accumulation of recombinant human epidermal growth factor (hEGF) in transgenic tobacco, a highly effective vector was constructed and transformed via Agrobacterium tumefaciens. The hEGF content in transgenic tobacco was up to 0.3% of the total soluble protein. Using the Vero E6 cell expansion assay and the MTT method for cell proliferation, hEGF produced by transgenic tobacco significantly stimulated Vero E6 cell expansion and proliferation, the same as commercial hEGF products.