Mesoderm patterning and somite formation during node regression: differential effects of chordin and noggin.
Mesoderm patterning and somite formation during node regression: differential effects of chordin and noggin.
复制标题
节点退化过程中的中胚层模式和体节形成：chordin 和 noggin 的差异效应。
DOI：
10.1016/s0925-4773(99)00085-4
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发表时间：
1999
影响因子：
2.6
通讯作者：
Stern,CD
中科院分区：
文献类型：
--
作者：
Streit,A;Stern,CD
在非洲爪哇，定义斯普曼组织器的特性之一是它有能力使中胚层背部。当被放置在预期的外侧/腹侧中胚层(血液、间充质)附近时，组织者使这些细胞采取更轴向/背侧的命运(肌肉)。高等脊椎动物胚胎的原始条纹似乎有类似的性质，但这一点尚未得到明确证明。利用鹌鹑/鸡的嵌合体和一组分子标记，我们证明了Hensen‘s结节(羊膜组织者)可以诱导后部原始条纹(预期的侧板)在早期神经元阶段形成体节(但不能形成脊索)。我们测试了两种BMP拮抗剂noggin和chordin(两者都在组织者中表达)，以确定它们从后部条纹产生体节和中间中胚层的能力，并发现noggin可以做到这一点，而不是chordin。相反，在发育早期，Chordin可以比noggin更有效地诱导异位原始条纹，而两种BMP拮抗剂都不能诱导胚胎外上皮细胞形成神经组织。神经形成伴随着结节的退化，这将预期的体节区域带入表达BMP-2、-4和-7的区域。在这一阶段，noggin的一个功能可能是保护未来的体节细胞免受BMPs的抑制作用。我们的结果表明，两种BMP拮抗剂noggin和chordin在羊膜发育的早期可能起不同的作用。
In Xenopus, one of the properties defining Spemann's organizer is its ability to dorsalise the mesoderm. When placed ajacent to prospective lateral/ventral mesoderm (blood, mesenchyme), the organizer causes these cells to adopt a more axial/dorsal fate (muscle). It seems likely that a similar property patterns the primitive streak of higher vertebrate embryos, but this has not yet been demonstrated clearly. Using quail/chick chimaeras and a panel of molecular markers, we show that Hensen's node (the amniote organizer) can induce posterior primitive streak (prospective lateral plate) to form somites (but not notochord) at the early neurula stage. We tested two BMP antagonists, noggin and chordin (both of which are expressed in the organizer), for their ability to generate somites and intermediate mesoderm from posterior streak, and find that noggin, but not chordin, can do this. Conversely, earlier in development, chordin can induce an ectopic primitive streak much more effectively than noggin, while neither BMP antagonist can induce neural tissue from extraembryonic epiblast. Neurulation is accompanied by regression of the node, which brings the prospective somite territory into a region expressing BMP-2, -4 and -7. One function of noggin at this stage may be to protect the prospective somite cells from the inhibitory action of BMPs. Our results suggest that the two BMP antagonists, noggin and chordin, may serve different functions during early stages of amniote development.