Sensory electrophysiology of bacteria: relationship of the membrane potential to motility and chemotaxis in Bacillus subtilis.
Sensory electrophysiology of bacteria: relationship of the membrane potential to motility and chemotaxis in Bacillus subtilis.
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细菌的感觉电生理学：枯草芽孢杆菌膜电位与运动性和趋化性的关系。
DOI：
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发表时间：
1977
影响因子：
11.1
通讯作者：
D. Koshland
中科院分区：
文献类型：
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作者：
J. B. Miller;D. Koshland
用一种菁染料的荧光探针检测了枯草芽孢杆菌的膜电位与运动性和趋化性的关系。染料荧光与三苯基甲基磷离子分布、厌氧和离子载体添加引起的变化有关，是膜电位的指示物。当电位足够运动并随时间保持恒定时，发现电位的绝对水平不影响细菌的游泳行为。然而，膜电位的短暂改变确实会导致游泳行为的改变。研究发现，引诱剂在不改变膜电位的情况下改变了细菌的游动行为。因此，正常枯草芽孢杆菌的整体膜电位的变化并不需要趋化性，但这种变化被细菌感知，就像感知引诱剂和驱虫剂水平的变化一样。
The relationship of membrane potential to motility and chemotaxis of Bacillus subtilis has been tested by using the fluorescence of a cyanine dye as a probe of the potential. The dye fluorescence was found to be an indicator of membrane potential by correlation with triphenylmethylphosphonium ion distribution and with changes due to anaerobicity and ionophore addition. When the potential was sufficient for motility and constant over time, it was found that the absolute level of the potential did not affect the swimming behavior of the bacteria. Transient alteration of the membrane potential did, however, lead to changes in swimming behavior. Attractants were found to alter the swimming behavior of the bacteria without altering the membrane potential. Thus, change of the overall membrane potential of a normal B. subtilis is not required for chemotaxis, but such a change is sensed by the bacteria just as changing levels of attractants and repellents are sensed.