The conserved HDAC Rpd3 drives transcriptional quiescence in S. cerevisiae.
The conserved HDAC Rpd3 drives transcriptional quiescence in S. cerevisiae.
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DOI：
10.1016/j.gdata.2015.10.008
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发表时间：
2015-12
期刊：
影响因子：
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通讯作者：
Tsukiyama T
中科院分区：
文献类型：
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作者：
McKnight JN;Tsukiyama T
静止是一个普遍存在的细胞周期阶段，从微生物到人类都保存下来，对正常细胞功能和对不断变化的环境条件的反应至关重要。我们最近报道了在酿酒酵母中与静止相关的大量抑制事件，其中Rpd3建立了抑制染色质结构，驱动转录关闭。在这里，我们详细描述了与出芽酵母转录静止特性相关的实验程序、数据收集和数据分析（GEO: GSE67151）。我们的研究结果提供了一个真正的分子事件，通过Rpd3的作用，由染色质结构的广泛变化驱动，将静止区分为酿酒酵母独特的细胞周期阶段。
Quiescence is a ubiquitous cell cycle stage conserved from microbes through humans and is essential to normal cellular function and response to changing environmental conditions. We recently reported a massive repressive event associated with quiescence in Saccharomyces cerevisiae, where Rpd3 establishes repressive chromatin structure that drives transcriptional shutoff. Here, we describe in detail the experimental procedures, data collection, and data analysis related to our characterization of transcriptional quiescence in budding yeast (GEO: GSE67151). Our results provide a bona fide molecular event driven by widespread changes in chromatin structure through action of Rpd3 that distinguishes quiescence as a unique cell cycle stage in S. cerevisiae.