LEUKOTRIENE-B4 IS A COMPLETE SECRETAGOGUE IN HUMAN-NEUTROPHILS - A KINETIC-ANALYSIS
LEUKOTRIENE-B4 IS A COMPLETE SECRETAGOGUE IN HUMAN-NEUTROPHILS - A KINETIC-ANALYSIS
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DOI：
10.1016/0006-291x(82)90622-2
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发表时间：
1982-01-01
影响因子：
3.1
通讯作者：
WEISSMANN, G
中科院分区：
文献类型：
--
作者：
SERHAN, CN;RADIN, A;WEISSMANN, G
本文研究了白三烯B_4（LTB_4）和20-COOH-LTB_4与人中性粒细胞（PMN）的相互作用。利用连续记录技术进行的动力学研究表明，LTB 4激活PMN的聚集、膜相关Ca 2+的动员，**图形 **。在暴露的几秒钟内产生和脱粒。剂量反应研究表明，LTB 4是更有效的比它的二羧酸衍生物（20-COOH-LTB 4）或其所有的反式异构体，并在很大程度上依赖于这些代理人的PMN响应细胞松弛素B的预处理的细胞。其性质与微生物离子载体离子霉素和A23187 [卡西霉素]的性质相似。LTB 4迅速激活PMN，并作为一个完整的促分泌素。氧化脂肪酸明显激活人PMN。
Interactions of leukotriene B4 (LTB4) and 20-COOH-LTB4 with human neutrophils (PMN) were studied. Kinetic studies, utilizing continuous recording techniques, showed that LTB4 activates PMN with respect to aggregation, mobilization of membrane-associated Ca2+, .**GRAPHIC**. generation, and degranulation within seconds of exposure. Dose-response studies indicate that LTB4 is much more potent than its dicarboxylic acid derivative (20-COOH-LTB4) or its all trans-isomer, and that PMN responses to these agents are largely dependent upon pretreatment of the cells with cytochalasin B. The properties were similar to those of the microbial ionophores, ionomycin and A23187 [calcimycin]. LTB4 rapidly activates PMN and serves as a complete secretagogue. Oxidized fatty acids apparently activate human PMN.