Characterization of Haemophilus parasuis isolated from Brazilian swine through serotyping, AFLP and PFGE
Characterization of Haemophilus parasuis isolated from Brazilian swine through serotyping, AFLP and PFGE
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DOI：
10.1016/j.rvsc.2011.04.006
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发表时间：
2012-06-01
影响因子：
2.4
通讯作者：
Moreno, Andrea Micke
中科院分区：
文献类型：
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作者：
Castilla, Karina Salvagni;Sena de Gobbi, Debora Dirani;Moreno, Andrea Micke
猪副猪嗜血杆菌感染的特征是纤维素性多浆膜炎、关节炎和脑膜炎。尽管传统的诊断是基于畜群历史、临床症状、细菌分离和血清分型，但基于分子的方法是物种特异性测试和流行病学研究的替代方法。本研究的目的是利用血清分型和基因分型方法对来自巴西不同州的副猪嗜血杆菌田间菌株进行特征分析。血清学分型以4型最多(26.1%)，其次为5型(17.4%)、14型(8.7%)、13型(4.4%)和2型(4.4%)，39%的菌株不能分型。使用单酶AFLP和PFGE可以对所有测试的菌株进行分型，分别产生34和20种不同的图谱，包括不可分型的菌株。除AFLP的鉴别指数稍高外，用Not I限制性内切酶对同一血清型、动物或农场来源的菌株进行分组，与流行病学资料有较好的相关性。结果表明，AFLP和PFGE是对巴西采集的副猪嗜血杆菌分离株进行分型的有价值的工具。(C)2011爱思唯尔有限公司。保留所有权利。
Haemophilus parasuis infection in pigs is characterized by fibrinous polyserositis, arthritis and meningitis. Despite the fact that traditional diagnosis is based on herd history, clinical signs, bacterial isolation and serotyping, molecular-based methods are alternatives for species-specific tests and epidemiological studies. The aim of this study was to characterize H. parasuis field strains from different states of Brazil, employing serotyping and genotyping methods. Serotyping revealed that serovar 4 was the most prevalent (26.1%), followed by serovars 5(17.4%), 14(8.7%), 13 (4.4%) and 2 (4.4%), whereas 39% of the strains were considered as untypeable. AFLP with a single enzyme and PFGE were able to type all isolates tested, generating 34 and 20 different profiles, respectively, including untypeable strains. Besides the slightly higher discrimination index presented by AFLP, PFGE with Not I restriction enzyme showed a better correlation with epidemiological data, grouping strains of the same serovar, animal or farm origin. The results indicated AFLP and PFGE as valuable tools for typing H. parasuis isolates collected in Brazil. (C) 2011 Elsevier Ltd. All rights reserved.