Microsatellite instability analysis using multiplex polymerase chain reaction with fluorescently labeled primers for amplification of five mononucleotide repeat markers
Microsatellite instability analysis using multiplex polymerase chain reaction with fluorescently labeled primers for amplification of five mononucleotide repeat markers
复制标题
使用多重聚合酶链反应和荧光标记引物进行微卫星不稳定性分析，用于扩增五个单核苷酸重复标记
DOI：
10.1111/hepr.13542
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发表时间：
2020
影响因子：
4.2
通讯作者：
Yuji Eso
中科院分区：
文献类型：
--
作者：
Yuta Tsugeno;Tadashi Yoshizawa;Yunyan Wu;Shintaro Goto;Toshihiro Haga;Takahiro Suzuki;Hiroko Seino;Satoko Morohashi;Hiroshi Kijima;Yuji Eso
I read with great interest the article by Kawaoka et al. and Ando et al. recently published in Hepatology Research and Clinical Journal of Gastroenterology, respectively. 1, 2 I appreciate the authors’ efforts to assess the incidence of microsatellite instability-high (MSI-H) hepatocellular carcinoma (HCC) among Japanese patients. The rate of MSI-H tumors in HCC has been reported to be very low (0–2.9%). 3 However, it would be significant to investigate the proportion of MSI-H tumors in Japanese patients, because MSI-H HCCs may respond to treatment with anti-programmed cell death-1 antibody including pembrolizumab. 4In their study, they examined the MSI status of HCC tissues in 82 patients (49 biopsy and 33 resection specimens) and their normal tissues using a multiplex polymerase chain reaction (PCR)-based companion diagnostic sequencing kit and identified that two of 82 patients (2.4%) were with MSI-H tumors. 1 The multiplex PCR-based MSI analysis is carried out using fluorescently labeled primers for amplification of five mononucleotide repeat markers (BAT-25, BAT-26, NR-21, NR-24, and MONO-27). 3 The PCR products were analyzed using specialized software developed for fragment analysis, such as GeneMapper Software. The X-axis represents the size in base pairs of the PCR products, and the Y-axis shows the intensity in relative fluorescence units. In MSI-H tumor tissue, the number of repetitions in each microsatellite region changes, resulting in a shift in the waveform peak, and another peak is found outside the quasi-monomorphic variation range (QMVR) of normal tissues (Fig. 1). The tumor is diagnosed as MSI-H if the waveform peaks of two or more microsatellite markers are shifted. In figure 1 of the article by Kawaoka et al. and figure 5 of the case report by Ando et al.(these are the same figures, case 1), the authors explained that the waveform peaks
