Local production of colony-stimulating factor within the bone marrow: role of nonhematopoietic cells.
Local production of colony-stimulating factor within the bone marrow: role of nonhematopoietic cells.
复制标题
骨髓内集落刺激因子的局部产生：非造血细胞的作用。
作者：
S. Chan;D. Metcalf
通过测定骨髓细胞或骨干在液体培养物中释放或产生活性物质的能力，评估小鼠骨髓腔内粒细胞和巨噬细胞调节剂集落刺激因子（CSF）的局部产生。在小鼠骨髓细胞的琼脂培养物中进行集落刺激活性测定。冲洗股骨骨髓细胞后，无论是否进行胰蛋白酶消化，骨中保留的骨髓细胞总数均低于5%。然而，由成年小鼠的这种骨形成的条件培养基的总集落刺激活性是由整个挤出的骨髓细胞群形成的条件培养基的8至20倍。新生小鼠股骨中的差异更为显著。这种活性的骨和骨髓细胞是抗辐射的。在全身照射后再生过程中，骨髓细胞活性保持不变，但干细胞相关细胞对条件培养基的能力显着上升，同时发生粒细胞和单核细胞祖细胞再生的开始。骨髓中非造血细胞局部产生CSF似乎是调节粒细胞生成和单核细胞形成的重要因素。
Local production of the granulocyte and macrophage regulator, colony-stimulating factor (CSF), within the mouse bone marrow cavity was assessed by determining the capacity of marrow cells or the bone shaft to release or produce active material in liquid cultures. Assays for colony-stimulating activity were performed in agar cultures of mouse bone marrow cells. Following flushing of femoral marrow cells, with or without trypsinization, less than 5% of total marrow cells remained in the bones. However, media conditioned by such bones from adult mice had eight to 20 times the total colony-stimulating activity of media conditioned by the entire extruded marrow cell population. The difference was more marked in femurs from neonatal mice. This activity of both bone and marrow cells was radioresistant. During regeneration following wholebody irradiation, marrow cell activity remained constant, but a significant rise in the capacity of shaft-related cells to conditioned medium occurred simultaneously with the onset of regeneration of granulocytic and monocytic progenitor cells. Local production of CSF by nonhematopoietic cells in the marrow appears to be a significant factor in regulating granulopoiesis and monocyte formation.