Composition of the Cockroach Gut Microbiome in the Presence of Parasitic Nematodes.
Composition of the Cockroach Gut Microbiome in the Presence of Parasitic Nematodes.
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DOI：
10.1264/jsme2.me16088
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发表时间：
2016-09-29
影响因子：
2.2
通讯作者：
Hasegawa K
中科院分区：
文献类型：
--
作者：
Vicente CS;Ozawa S;Hasegawa K
蟑螂是由线虫寄生的，这种线虫以专属的方式生活在它们的后肠里，并与居住的微生物群落相互作用。本研究通过对黑胸大蠊和美洲大蠊肠道微生物群的组成分析，研究了黑腹大蠊和美洲大蠊肠道线虫的自然感染和人工感染。制备了9个16S rRNA基因V3-V4区文库，用于焦磷酸测序。我们检测了自然感染附属莱德线虫和无线虫的实验室饲养的黑胸拟青霉的完整肠道微生物群(前、中、后肠道)，并通过表征自然感染Diesingi Hammerschmidtiella和Thelastoma Bulhoesi的实验室饲养的美洲对虾和人工感染附着线虫的美洲对虾的后肠微生物群落来补充我们的研究。结果表明，自然感染和无线虫感染的褐飞虱前肠和中肠微生物群落接近，与后肠微生物群落相反；两种蟑螂的后肠微生物群落在有自然寄生虫存在的情况下表现出更高的微生物多样性，最具代表性的类群丰度存在显著差异，分别是Firmicuts、变形杆菌和拟杆菌。我们的结果提供了基本信息，并鼓励进一步研究蟑螂肠道以及在这种环境中发挥作用的线虫的多营养相互作用。
Cockroaches are parasitized by thelastomatid nematodes, which live in an obligate manner in their hindgut and interact with the resident microbial community. In the present study, a composition analysis was performed on the gut microbiome of Periplaneta fuliginosa and P. americana to investigate natural and artificial infection by thelastomatid nematodes. Nine libraries of the 16S rRNA gene V3–V4 region were prepared for pyrosequencing. We examined the complete gut microbiome (fore-, mid-, and hindgut) of lab-reared P. fuliginosa naturally infected with the parasitic nematode Leidynema appendiculatum and those that were nematode-free, and complemented our study by characterizing the hindgut microbial communities of lab-reared P. americana naturally infected with Hammerschmidtiella diesingi and Thelastoma bulhoesi, artificially infected with L. appendiculatum, and those that were nematode-free. Our results revealed that the fore- and midgut of naturally infected and nematode-free P. fuliginosa have close microbial communities, which is in contrast with hindgut communities; the hindgut communities of both cockroaches exhibit higher microbial diversities in the presence of their natural parasites and marked differences were observed in the abundance of the most representative taxa, namely Firmicutes, Proteobacteria, and Bacteroidetes. Our results have provided basic information and encourage further studies on multitrophic interactions in the cockroach gut as well as the thelastomatid nematodes that play a role in this environment.