Selective loss of the suppressor-inducer T-cell subset in progressive multiple sclerosis. Analysis with anti-2H4 monoclonal antibody.
Selective loss of the suppressor-inducer T-cell subset in progressive multiple sclerosis. Analysis with anti-2H4 monoclonal antibody.
复制标题
进行性多发性硬化症中抑制-诱导 T 细胞亚群的选择性丧失。
DOI：
10.1056/nejm198701083160202
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Schlossman,SF
中科院分区：
文献类型：
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作者：
Morimoto,C;Hafler,DA;Weiner,HL;Letvin,NL;Hagan,M;Daley,J;Schlossman,SF
T4+淋巴细胞群包括诱导抑制性T淋巴细胞（T8+细胞）的亚群，并且可以通过使用抗2 H4和抗T4单克隆抗体的双色荧光分析来区分。为了研究这些细胞在多发性硬化中的可能作用，我们使用抗2 H4抗体来表征63例进行性、稳定性或急性（复发-缓解型）多发性硬化患者的外周血淋巴细胞亚群。37例进行性多发性硬化患者中有23例外周血T细胞诱导抑制细胞（T4+ 2 H4+细胞）的数量和百分比选择性下降，而16例稳定期患者中只有3例和10例急性发作患者中只有2例显著下降。这些循环T4+ 2 H4+细胞的选择性减少仅发生在34名患有其他神经系统疾病的患者对照中的1名和50名健康对照中的2名（通过Fisher精确检验，P<0.0001）。研究人群中T4+ 2 H4+细胞的绝对数量和反应性百分比在进行性多发性硬化患者中为187±28/mm 3和8.3± 1%;在稳定性疾病患者中为353±60/mm 3和14.5± 2%;在急性疾病患者中为368±72和14.6± 2.1%;其他神经系统疾病对照组为402±64和15.6± 2%;健康对照组为519±44和19.7± 1%。使用美洲商陆丝裂原驱动的IgG测定的功能研究证明了体外T4+ 2 H4+细胞数量减少和IgG产量增加之间的相关性。家族研究表明2 H4抗原不是遗传多态性抗原决定簇的一部分，我们的研究结果表明，在进行性多发性硬化症中，抑制性T细胞诱导物的减少可能允许与中枢神经系统元素反应的细胞活化。（N Engl J Med 1987; 316：67-72.）
The T4+ lymphocyte population includes a subset that induces suppressor T lymphocytes (T8+ cells) and can be distinguished by dual-color fluorescence analysis with anti-2H4 and anti-T4 monoclonal antibodies. To investigate the possible role of these cells in multiple sclerosis, we used anti-2H4 antibody to characterize peripheral-blood lymphocyte subsets in 63 patients with multiple sclerosis that was progressive, stable, or acute (relapsing–remitting). Twenty-three of 37 patients with progressive multiple sclerosis had a selective decrease in the number and percentage of peripheral-blood T cells that induce suppressor cells (T4+2H4+ cells), whereas only 3 of 16 patients with stable disease and 2 of 10 patients in the midst of an acute attack had a significant decrease. These selective decreases of circulating T4+2H4+ cells occurred in only 1 of 34 patient controls with other neurologic diseases and in 2 of 50 healthy controls (P<0.0001 by Fisher's exact test). The absolute number of T4+2H4+ cells and the percentage of reactivity in the populations studied were 187±28 per cubic millimeter and 8.3±1 percent in patients with progressive multiple sclerosis; 353±60 per cubic millimeter and 14.5±2 percent in patients with stable disease; 368±72 and 14.6±2.1 percent in patients with acute disease; 402±64 and 15.6±2 percent in controls with other neurologic diseases; and 519±44 and 19.7±1 percent in healthy controls. Functional studies using a pokeweed mitogen–driven IgG assay demonstrated a correlation between decreased numbers of T4+2H4+ cells and increased production of IgG in vitro. Family studies showed that the 2H4 antigen was not part of an inherited polymorphic antigenic determinant.Our results suggest that in progressive multiple sclerosis decreases in inducers of suppressor T cells may permit the activation of cells reactive with elements of the central nervous system. (N Engl J Med 1987; 316:67–72.)