Ftx is dispensable for imprinted X-chromosome inactivation in preimplantation mouse embryos.
Ftx is dispensable for imprinted X-chromosome inactivation in preimplantation mouse embryos.
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Ftx 对于植入前小鼠胚胎中的印记 X 染色体失活是可有可无的。
DOI：
10.1038/srep05181
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发表时间：
2014-06-05
影响因子：
4.6
通讯作者：
Kobayashi S
中科院分区：
文献类型：
--
作者：
Soma M;Fujihara Y;Okabe M;Ishino F;Kobayashi S
在雌性哺乳动物中，X染色体失活（XCI）通过使两条X染色体中的一条失活来平衡两性之间的基因表达。Xist被认为是一个主要的顺式作用因子，可使着床前小鼠胚胎（印迹XCI）中父系来源的X染色体（Xp）失活。Ftx被认为是Xist的积极监管者。然而，Ftx在雌性动物中的生理作用从未被研究过。我们最近报道Ftx位于印迹XCI的顺式调控区域，并由非活性Xp表达，这表明Ftx在印迹XCI机制中起作用。在这里，我们研究了靶向删除Ftx对印迹XCI的影响。Ftx的破坏不影响雌性胚胎的存活，也不影响着床前雌性胚胎中Xist等x连锁基因的表达。我们的结果表明，Ftx对于着床前胚胎的印迹XCI是不可缺少的。
X-chromosome inactivation (XCI) equalizes gene expression between the sexes by inactivating one of the two X chromosomes in female mammals. Xist has been considered as a major cis-acting factor that inactivates the paternally derived X chromosome (Xp) in preimplantation mouse embryos (imprinted XCI). Ftx has been proposed as a positive regulator of Xist. However, the physiological role of Ftx in female animals has never been studied. We recently reported that Ftx is located in the cis-acting regulatory region of the imprinted XCI and expressed from the inactive Xp, suggesting a role in the imprinted XCI mechanism. Here we examined the effects on imprinted XCI using targeted deletion of Ftx. Disruption of Ftx did not affect the survival of female embryos or expression of Xist and other X-linked genes in the preimplantation female embryos. Our results indicate that Ftx is dispensable for imprinted XCI in preimplantation embryos.