Comparative proteomics of Mesembryanthemum crystallinum guard cells and mesophyll cells in transition from C3 to CAM
Comparative proteomics of Mesembryanthemum crystallinum guard cells and mesophyll cells in transition from C3 to CAM
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DOI：
10.1016/j.jprot.2020.104019
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发表时间：
2021-01-16
影响因子：
3.3
通讯作者：
Chen, Sixue
中科院分区：
文献类型：
--
作者：
Guan, Qijie;Kong, Wenwen;Chen, Sixue
盐胁迫可诱导中胚草将其光合作用从C-3代谢转移到景天酸代谢（CAM），从而提高植物水分利用效率。研究M。堆积的铀改变了其碳固定途径，这对于潜在地转化为作物和增强作物的适应性是重要的。在这项研究中，我们研究了蛋白质组的变化，保卫细胞和叶肉细胞的过程中的C-3 CAM过渡。我们收集了丰富的保卫细胞和叶肉细胞在一个短暂的过渡期。在两种细胞类型中共鉴定和定量了1153种蛋白质。在转化过程中，保卫细胞和叶肉细胞中的蛋白质发生了不同程度的变化。例如，我们观察到叶肉细胞中的夜间碳固定和两种细胞类型中参与细胞生长的蛋白质。渗透调节、离子转运、能量代谢和光反应等相关蛋白在C3向CAM的转化过程中起重要作用。进行实时PCR实验以确定转录物和蛋白质水平之间的潜在相关性。这些结果突出了潜在的分子机制的重要兼性CAM植物的保卫细胞和叶肉细胞的C-3 CAM转换。生物学意义：淡水资源农业食品生产是一个全球性的挑战。大自然已经进化出具有增强的水利用效率的景天科酸代谢（CAM）植物。利用单细胞型蛋白质组学技术，揭示了冰草光合作用由C-3向CAM转变过程中保卫细胞和叶肉细胞的分子变化。这些结果为CAM过渡提供了重要的见解，并可能有助于提高作物对全球粮食安全的适应能力。
Salinity can induce Mesembryanthemum crystallinum to shift its photosynthesis from C-3 to crassulacean acid metabolism (CAM), leading to enhanced plant water use efficiency. Studying how M. crystallinum changes its carbon fixation pathways is important for potential translation into crops and enhancing crop resilience. In this study, we examined proteomic changes in guard cells and mesophyll cells in the course of the C-3 to CAM transition. We collected enriched guard cells and mesophyll cells during a short period of transition. A total of 1153 proteins were identified and quantified in the two cell-types. During the transition, proteins in the guard cells and mesophyll cells exhibited differential changes. For example, we observed nocturnal carbon fixation in mesophyll cells and proteins involved in cell growth in the two cell-types. Proteins involved in osmotic adjustment, ion transport, energy metabolism and light response may play important roles in the C-3 to CAM transition. Real-time PCR experiments were conducted to determine potential correlations between transcript and protein levels. These results have highlighted potential molecular mechanisms underlying the C-3 to CAM transition of guard cells and mesophyll cells of the important facultative CAM plant.Biological significance: Fresh water resource for agricultural food production is a global challenge. Nature has evolved crassulacean acid metabolism (CAM) plants with enhanced water use efficiency. Using single cell-type proteomics, this study revealed molecular changes taking place in guard cells and mesophyll cells during the shift of ice plant photosynthesis from C-3 to CAM. The results have provided important insights into the CAM transition and may facilitate effort toward enhancing crop resilience for global food security.