Dynamic actin polymerization drives T cell receptor-induced spreading: A role for the signal transduction adaptor LAT
Dynamic actin polymerization drives T cell receptor-induced spreading: A role for the signal transduction adaptor LAT
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DOI：
10.1016/s1074-7613(01)00112-1
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发表时间：
2001-03-01
期刊：
影响因子：
32.4
通讯作者：
Samelson, LE
中科院分区：
文献类型：
--
作者：
Bunnell, SC;Kapoor, V;Samelson, LE
T细胞活化诱导细胞形状和细胞骨架结构的功能变化。为了便于收集活化T细胞的动态高分辨率图像，我们将T细胞接种在涂有T细胞受体（TCR）抗体的盖玻片上。使用这些图像，我们能够定量单个细胞随时间的形态反应。在这里，我们表明，TCR接合触发的形成和扩展的接触不断重塑肌动蛋白丰富的环，这些过程与延长板状伪足，并需要肌动蛋白聚合，酪氨酸激酶激活，细胞质钙增加，和LAT，一个重要的造血适配器。此外，维持所产生的接触需要持续的钙流入，完整的微管细胞骨架和功能性LAT。
T cell activation induces functional changes in cell shape and cytoskeletal architecture. To facilitate the collection of dynamic, high-resolution images of activated T cells, we plated T cells on coverslips coated with antibodies to the T cell receptor (TCR). Using these images, we were able to quantitate the morphological responses of individual cells over time. Here, we show that TCR engagement triggers the formation and expansion of contacts bounded by continuously remodeled actin-rich rings, These processes are associated with the extension of lamellipodia and require actin polymerization, tyrosine kinase activation, cytoplasmic calcium increases, and LAT, an important hematopoietic adaptor. In addition, the maintenance of the resulting contact requires sustained calcium influxes, an intact microtubule cytoskeleton, and functional LAT.