PRR1, a homolog of Aspergillus nidulans palF, controls pH-dependent gene expression and filamentation in Candida albicans
PRR1, a homolog of Aspergillus nidulans palF, controls pH-dependent gene expression and filamentation in Candida albicans
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DOI：
10.1128/jb.181.24.7516-7523.1999
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发表时间：
1999-12-01
影响因子：
3.2
通讯作者：
Fonzi, WA
中科院分区：
文献类型：
--
作者：
Porta, A;Ramon, AM;Fonzi, WA
环境 pH 值与控制酵母-菌丝转变和白色念珠菌的发病机制有关。白色念珠菌的几个基因，包括 PHR1 和 PHR2，其表达依赖于 pH 值。为了研究 pH 依赖性表达的机制，我们克隆并表征了 PRR1（pH 响应调节剂）。 PRR1 与 palF 同源，palF 是构巢曲霉 pH 响应途径的一个组成部分，PRR1 的表达本身是 pH 依赖性的，在酸性 pH 下达到最大，但在碱性 pH 下降低数倍。在 prr1 null 突变体中，碱诱导的 PHR1 表达被完全消除。相反，PHR2 的表达在碱性 pH 值下不再受到抑制。 prr1 缺失突变体在任一 pH 值下均未表现出形态异常；然而，它在培养基199和10%血清平板上失去了形成菌丝的能力。PHRI的强制表达并没有恢复在血清上形成丝的能力，这表明菌丝发育需要额外的PRR1依赖性基因。这些发育基因似乎与发育调节因子 EFG1 控制的基因不同，因为 EFG1 依赖性基因 HWP1 在 prr1 无效突变体中正常表达。我们得出结论，PRR1 编码白色念珠菌中 pa 依赖性反应途径的一个组成部分，并且该途径调节菌丝发育的多个组成部分的表达。
The pH of the environment has been implicated in controlling the yeast-hypha transition and pathogenesis of Candida albicans, Several C, albicans genes, including PHR1 and PHR2, are pH dependent in their expression. To investigate the mechanism of pH-dependent expression, we have cloned and characterized PRR1 (for pH response regulator). PRR1 is homologous to palF, a component of the pH response pathway in Aspergillus nidulans, Expression of PRR1 was itself pH dependent, being maximal at acid pH hut reduced severalfold at alkaline pH, In a prr1 null mutant the alkaline-induced expression of PHR1 was completely abolished. Conversely, expression of PHR2 was no longer repressed at alkaline pH. A prr1 null mutant exhibited no morphological abnormalities at either pH; however, it lost the ability to form hyphae on medium 199 and on 10% serum plates, The ability to filament on serum was not restored by forced expression of PHRI, indicating that additional PRR1-dependent genes are required for hyphal development. These developmental genes appear to be distinct from those controlled by the developmental regulator EFG1, since the EFG1-dependent gene HWP1 was expressed normally in the prr1 null mutant. We conclude that PRR1 encodes a component of the pa-dependent response pathway in C. albicans and that this pathway regulates the expression of multiple components of hyphal development.