REGENERATING SKELETAL-MUSCLE CELLS EXPRESS INSULIN-LIKE GROWTH FACTOR-I
REGENERATING SKELETAL-MUSCLE CELLS EXPRESS INSULIN-LIKE GROWTH FACTOR-I
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DOI：
10.1111/j.1748-1716.1987.tb08144.x
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发表时间：
1987-06-01
期刊：
影响因子：
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通讯作者：
HANSSON, HA
中科院分区：
文献类型：
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作者：
JENNISCHE, E;HANSSON, HA
本实验观察了营养肽胰岛素样生长因子-I(IGF-I)在太保新伤小鼠比目鱼肌再生过程中的表达。在对照条件下，肌肉细胞中没有观察到特异性的IGF-I免疫反应。注射太保新后2天内，激活的卫星细胞内可见IGF-I免疫反应阳性。成肌细胞和肌管表达高IGF-I免疫反应性。IGF-I免疫反应呈胞浆阳性，明显与多聚核糖体相关。未见泡状或膜状IGF-I免疫反应阳性。结论：IGF-I是在骨骼肌再生过程中由肌源性细胞合成的。提示IGF-I主要通过自分泌机制作用于骨骼肌。
The expression of the trophic peptide insulin‐like growth factor I (IGF‐I; somatomedin C) was investigated in the regenerating soleus muscle of mice after injury by the snake venom taipoxin. No specific IGF‐I immunoreactivity was observed in muscle cells during control conditions. Within 2 days after taipoxin injection, IGF‐I immunoreactivity could be demonstrated in activated satellite cells. Myoblasts and myotubes expressed high IGF‐I immunoreactivity. The IGF‐I immunoreactivity was strictly cyto‐plasmatic and obviously associated with polyribosomes. No vesicular or membraneous IGF‐I immunoreactivity could be demonstrated. It is concluded that IGF‐I is synthesized in myogenic cells during skeletal muscle regeneration. It is suggested that IGF‐I exerts its effects on skeletal muscle mainly by autocrine mechanisms.