A novel family of mammalian taste receptors
A novel family of mammalian taste receptors
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DOI：
10.1016/s0092-8674(00)80705-9
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发表时间：
2000-03-17
期刊：
影响因子：
64.5
通讯作者：
Zuker, CS
中科院分区：
文献类型：
--
作者：
Adler, E;Hoon, MA;Zuker, CS
在哺乳动物中，味觉感知是感觉输入的主要模式。我们已经确定了一个新的家庭的40-80人类和啮齿动物的G蛋白偶联受体表达的味觉受体细胞的舌头和腭上皮细胞的子集。这些候选味觉受体（T2 R）在基因组中以簇的形式组织，并与影响小鼠和人类苦味感知的基因座遗传相关。值得注意的是，单个味觉受体细胞表达大量的T2 R，这表明每个细胞都能够识别多种促味剂。T2 R仅在含有G蛋白α亚基gustducin的味觉受体细胞中表达，这意味着它们作为gustducin连接的受体起作用。在随附的论文中，我们证明了T2 Rs在体外与味蛋白偶联，并在功能表达测定中对苦味促味剂作出反应。
In mammals, taste perception is a major mode of sensory input. We have identified a novel family of 40-80 human and rodent G protein-coupled receptors expressed in subsets of taste receptor cells of the tongue and palate epithelia. These candidate taste receptors (T2Rs) are organized in the genome in clusters and are genetically linked to loci that influence bitter perception in mice and humans. Notably, a single taste receptor cell expresses a large repertoire of T2Rs, suggesting that each cell may be capable of recognizing multiple tastants. T2Rs are exclusively expressed in taste receptor cells that contain the G protein alpha subunit gustducin, implying that they function as gustducin-linked receptors. In the accompanying paper, we demonstrate that T2Rs couple to gustducin in vitro, and respond to bitter tastants in a functional expression assay.