The effects of graded levels of calorie restriction: II. Impact of short term calorie and protein restriction on circulating hormone levels, glucose homeostasis and oxidative stress in male C57BL/6 mice.
The effects of graded levels of calorie restriction: II. Impact of short term calorie and protein restriction on circulating hormone levels, glucose homeostasis and oxidative stress in male C57BL/6 mice.
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DOI：
10.18632/oncotarget.4003
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发表时间：
2015-09-15
期刊：
影响因子：
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通讯作者：
Speakman JR
中科院分区：
文献类型：
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作者：
Mitchell SE;Delville C;Konstantopedos P;Hurst J;Derous D;Green C;Chen L;Han JJ;Wang Y;Promislow DE;Lusseau D;Douglas A;Speakman JR
限制食物摄入量可以减弱衰老的许多有害影响，影响健康寿命并延长寿命。究竟是卡路里的总体限制（卡路里限制：CR）还是蛋白质等常量营养素的偶然减少（蛋白质限制：PR）介导了这些影响尚不清楚。将3个月CR或PR（10 - 40%）对C57 BL/6小鼠的影响与自由进食的对照组进行比较。循环中瘦素、肿瘤坏死因子-α和胰岛素样生长因子-1（IGF-1）的减少与CR水平相关，并与形态学改变相关，但在PR后保持不变。CR后葡萄糖耐量和胰岛素敏感性改善，但不受PR影响。PR没有改变氧化应激的生物标志物。CR显著降低了主要尿蛋白的水平，表明生殖投资降低。这里的结果支持这样的观点，即降低脂肪因子水平，改善胰岛素/IGF-1信号传导和减少生殖投资在CR的有益作用中起重要作用，而在短期内，氧化损伤的减弱是不适用的。PR没有复制任何积极效果。
Limiting food intake attenuates many of the deleterious effects of aging, impacting upon healthspan and leading to an increased lifespan. Whether it is the overall restriction of calories (calorie restriction: CR) or the incidental reduction in macronutrients such as protein (protein restriction: PR) that mediate these effects is unclear. The impact of 3 month CR or PR, (10 to 40%), on C57BL/6 mice was compared to controls fed ad libitum. Reductions in circulating leptin, tumor necrosis factor-α and insulin-like growth factor-1 (IGF-1) were relative to the level of CR and individually associated with morphological changes but remained unchanged following PR. Glucose tolerance and insulin sensitivity were improved following CR but not affected by PR. There was no indication that CR had an effect on oxidative damage, however CR lowered antioxidant activity. No biomarkers of oxidative stress were altered by PR. CR significantly reduced levels of major urinary proteins suggesting lowered investment in reproduction. Results here support the idea that reduced adipokine levels, improved insulin/IGF-1 signaling and reduced reproductive investment play important roles in the beneficial effects of CR while, in the short-term, attenuation of oxidative damage is not applicable. None of the positive effects were replicated with PR.