Oral administration with attenuated Salmonella encoding a Trichinella cystatin-like protein elicited host immunity
Oral administration with attenuated Salmonella encoding a Trichinella cystatin-like protein elicited host immunity
复制标题
口服编码旋毛虫半胱氨酸蛋白酶抑制剂样蛋白的减毒沙门氏菌可引发宿主免疫
DOI：
10.1016/j.exppara.2014.03.015
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发表时间：
2014-06-01
影响因子：
2.1
通讯作者：
Liu, M. Y.
中科院分区：
文献类型：
--
作者：
Liu, X. D.;Wang, X. L.;Liu, M. Y.
旋毛虫病是一个公共卫生问题，在许多国家被视为一种新发/再发疾病。利用实验感染2万只旋毛虫肌肉幼虫的猪血清免疫筛选肠道肌肉幼虫cDNA文库，鉴定了一种胱抑素样蛋白（ts -胱抑素）。为了研究其对宿主免疫的影响，我们在比较两种鼠伤寒沙门菌给药方案的免疫原性的基础上，选择了真核表达系统，结果表明真核表达系统更优越。检测体液IgG和粘膜IgA抗体反应。为了探究Th1和Th2反应是否对诱导保护起作用，我们检测了Th1和Th2特异性细胞转录因子和细胞因子谱。流式细胞术检测T淋巴细胞和巨噬细胞群的变化。最后进行寄生虫学检查。结果表明，Ts-cystatin诱导Th1/ th2混合型免疫应答，降低STAT6转录。ts -胱抑素组肠道成虫恢复率提高10.9%，繁殖率降低91%。此外，与对照组相比，肌肉幼虫的数量没有变化。综上所述，我们的研究结果表明，ts -胱抑素在旋毛虫抵抗宿主快速驱逐的过程中发挥了重要作用，值得进一步研究。(C) 2014爱思唯尔公司版权所有。
Trichinellosis is a public health problem and is regarded as an emergent/re-emergent disease in various countries. The cDNA encoding a cystatin-like protein (Ts-cystatin) was identified by immunoscreening intestinal muscle larvae cDNA libraries with serum from pigs experimentally infected with 20,000 Trichinella spiralis muscle larvae. To study its impact on host immunity, we chose a eukaryotic expression system based on several comparisons of immunogenicity between the two Salmonella typhimurium administration schemes, which indicated that the eukaryotic expression system was superior. Humoral IgG and mucosal IgA were measured to determine the antibody response. To explore whether Th1 and Th2 responses were responsible for the induced protection, Th1- and Th2-specific cellular transcription factors and the cytokine profile were examined. Changes in the T lymphocyte and macrophage populations were detected by flow cytometry. Lastly, parasitological examination was examined. The results showed that Ts-cystatin induced a Th1/Th2-mixed type of immune response and decreased STAT6 transcription. The intestinal adult recovery increased by 10.9% in the Ts-cystatin group, the Ts-cystatin group fecundity rate was decreased by 91%. Furthermore, the number of muscle larvae did not change compared with the control group. In conclusion, our results suggest that Ts-cystatin plays an important role in Trichinella resistance to rapid expulsion by the host and is worth further study. (C) 2014 Elsevier Inc. All rights reserved.