Molecular characterization of unusual human G5P[6] rotaviruses identified in China
Molecular characterization of unusual human G5P[6] rotaviruses identified in China
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DOI：
10.1016/j.jcv.2007.12.013
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发表时间：
2008-06-01
影响因子：
8.8
通讯作者：
Fang, Zhao-yin
中科院分区：
文献类型：
--
作者：
Li, Dan-di;Duan, Zhao-jun;Fang, Zhao-yin
背景：我们在中国河北省卢龙县收集的粪便样本中发现了一种不寻常的人类轮状病毒，G5P[6]基因型的LL36755。这是亚洲首次检测到 G5 血清型轮状病毒。 目的： 鉴定和表征从 5 岁以下急性胃肠炎儿童收集的 988 份粪便样本中的 G5 轮状病毒。 研究设计： 我们使用 G5 基因型特异性引物，通过 RT-PCR 分析了 459 份轮状病毒阳性样本。对 G5 菌株进行了测序。结果：鉴定出另外两个 G5 阳性样本（LL3354 和 LL4260）。对LL3354、LL4260和LL36755菌株的VP7、VP4、VP6和NSP4基因进行了测序。 VP4 序列与猪 P[6] 品系组成一组。 LL3354和LL36755株的VP6序列在系统发育上分别接近SGI和SGII轮状病毒的主要簇。推导的LL4260株VP6蛋白具有SGI和SGII株的特征，但最适合非典型SGI病毒簇。此外，根据NSP4序列，三个G5毒株属于基因组B，与人毒株Wa最接近。结论：这些结果表明人和猪轮状病毒之间存在动态相互作用，并表明重配可以导致猪基因等位基因稳定引入并成功扩散到人轮状病毒中。 (c) 2008 Elsevier B.V. 保留所有权利。
Background: We found an unusual human rotavirus, LL36755 of G5P[6]genotype, in a stool sample collected in Lulong County, Hebei Province, China. This is the first detection of rotavirus serotype G5 in Asia.Objectives: To identify and characterize G5 rotaviruses in 988 stool samples collected from children under 5 years old with acute gastroenteritis.Study design: We analyzed 459 rotavirus-positive samples with RT-PCR using G5 genotype-specific primers. The G5 strains were sequenced.Results: Two additional G5-positive samples (LL3354 and LL4260) were identified. VP7, VP4, VP6 and NSP4 genes of LL3354, LL4260 and LL36755 strains were sequenced. The VP4 sequences formed a group with porcine P[6] strains. The VP6 sequences of strains LL3354 and LL36755 were phylogenetically close to the major clusters of SGI and SGII rotaviruses, respectively. The deduced VP6 protein of strain LL4260 had characteristics of both SGI and SGII strains, but best fit with a cluster of atypical SGI viruses. In addition, based on NSP4 sequences, the three G5 strains belonged to genogroup B and were closest to human strain Wa.Conclusion: These results indicate a dynamic interaction of human and porcine rotaviruses and suggest that reassortment could result in the stable introduction and successful spread of porcine gene alleles into human rotaviruses. (c) 2008 Elsevier B.V. All rights reserved.