Identification in culture of a class of hemopoietic colony-forming units with extensive capability to self-renew and generate multipotential hemopoietic colonies.
Identification in culture of a class of hemopoietic colony-forming units with extensive capability to self-renew and generate multipotential hemopoietic colonies.
复制标题
在培养物中鉴定一类具有广泛的自我更新能力和产生多潜能造血集落的造血集落形成单位。
DOI：
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发表时间：
1982
影响因子：
11.1
通讯作者：
M. Ogawa
中科院分区：
文献类型：
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作者：
T. Nakahata;M. Ogawa
在甲基纤维素培养物中，在商陆丝裂原刺激的小鼠脾细胞条件培养基中孵育 16 天后，小鼠骨髓和脾细胞形成由 40-1,000 个母细胞组成的集落。这些集落可以通过完全不存在终末分化的迹象而与其他造血集落原位区分。这些集落（暂定名为干细胞集落）的重新铺板揭示了它们的自我更新能力和产生次级集落的广泛能力，其中许多是多能造血集落。一些菌落显示出 100% 的重铺效率。对单个干细胞集落的分析显示，培养物中脾集落形成单位和肉眼可见的粒细胞-红细胞-巨噬细胞-巨核细胞集落形成单位（CFU-GEMM）同时存在且发生率较高。干细胞集落和GEMM集落之间的重新铺板比较强烈表明，干细胞集落的祖细胞在干细胞分化的层次中比CFU-GEMM更高。干细胞集落的定量提供了对此处描述的原始造血祖细胞类别的测定。
Mouse marrow and spleen cells formed colonies consisting of 40-1,000 blast cells after 16 days of incubation in methylcellulose culture in the presence of medium conditioned by pokeweed mitogen-stimulated mouse spleen cells. These colonies could be distinguished from other hemopoietic colonies in situ by the complete absence of signs of terminal differentiation. Replating of these colonies (tentatively named stem cell colonies) revealed their self-renewal capacity and the extensive ability to generate secondary colonies, many of which were multipotential hemopoietic colonies. Some of the colonies revealed 100% replating efficiencies. Analyses of individual stem cells colonies revealed concurrent and high incidences of spleen colony-forming units and the macroscopic granulocyte-erythrocyte-macrophage-megakaryocyte colony-forming units (CFU-GEMM) in culture. Replating comparison between the stem cell colonies and GEMM colonies strongly indicated that the progenitors for the stem cell colonies are higher in the hierarchy of stem cell differentiation than are CFU-GEMM. Quantitation of stem cell colonies provides an assay for the class of primitive hemopoietic progenitors described here.