Evolution of the albumin: alpha-fetoprotein ancestral gene from the amplification of a 27 nucleotide sequence.
Evolution of the albumin: alpha-fetoprotein ancestral gene from the amplification of a 27 nucleotide sequence.
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白蛋白的进化：甲胎蛋白祖先基因，来自 27 个核苷酸序列的扩增。
DOI：
10.1016/0022-2836(84)90187-6
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发表时间：
1984
影响因子：
5.6
通讯作者：
Tilghman,SM
中科院分区：
文献类型：
--
作者：
Alexander,F;Young,PR;Tilghman,SM
甲胎蛋白和白蛋白的基因是由一个包含三个遗传结构域的祖先基因的复制产生的。这些结构域是由五个外显子或亚结构域组成的原始遗传结构域的三倍化产生的。小鼠甲胎蛋白基因各亚结构域之间的核苷酸序列同源性表明，原始结构域本身是由一个更简单的序列扩增而产生的。对这些同源性的详细分析表明，五个亚结构域家族中的每一个都含有27个碱基长的重复序列的残余，整个甲胎蛋白编码序列已从该重复序列组装而成。推导出27个核苷酸重复序列的共有序列，并描述了每个子域内重复序列的位置。提出了一个模型的原始结构域的进化的扩增和分歧的27个碱基对序列，沿着与缩合的重复到子域分隔的插入序列。据推测，插入序列的作用可能是限制基因（如甲胎蛋白和白蛋白）的序列扩增，这些基因的蛋白质产物不能耐受大小变化。
The genes for α-fetoprotein and albumin arose by duplication of an ancestral gene that contained three genetic domains. These domains were generated by the triplication of a primordial genetic domain composed of five exons or subdomains. That the primordial domain itself arose by amplification of a simpler sequence is suggested by nucleotide sequence homologies among the subdomains of the mouse α-fetroprotein gene. A detailed analysis of these homologies reveals that each of the five subdomain families contains remnants of a 27-base-long repeat from which the entire α-fetoprotein coding sequence has been assembled. A consensus sequence for the 27 nucleotide repeat is derived, and the positions of the repeats within each subdomain are described. A model is proposed for the evolution of the primordial domain by the amplification and divergence of the 27 base-pair sequence, along with the condensation of the repeats into subdomains separated by intervening sequences. It is postulated that the role of intervening sequences may be to limit sequence amplification in genes such as a-fetoprotein and albumin whose protein products cannot tolerate size variation.