Monitoring gastric lymphoma in peripheral blood by quantitative IgH allele‐specific oligonucleotide real‐time PCR and API2‐MALT1 PCR
Monitoring gastric lymphoma in peripheral blood by quantitative IgH allele‐specific oligonucleotide real‐time PCR and API2‐MALT1 PCR
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通过定量 IgH 等位基因特异性寡核苷酸实时 PCR 和 API2-MALT1 PCR 监测外周血胃淋巴瘤
DOI：
10.1111/j.1365-2141.2005.05807.x
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发表时间：
2005
影响因子：
6.5
通讯作者：
J. V. Krieken
中科院分区：
文献类型：
--
作者：
Max I. Schreuder;Marieke A. Hoeve;L. Groothuis;Henk Boot;L. Boerrigter;D. Jong;R. A. Veenendaal;J. Jansen;J. V. Krieken
胃结外边缘区淋巴瘤（EMZL）通常表现出长期的局部疾病，但本研究证明低阶段患者的外周血（PB）中存在肿瘤细胞。我们通过实时免疫球蛋白 (Ig)H 等位基因特异性寡核苷酸聚合酶链反应 (ASO-PCR) 和 API2-MALT1 PCR 研究了携带或缺乏 t(11;18)(q21;q21) 的胃淋巴瘤患者 PB 中肿瘤细胞的存在。仅在 t(11;18)(q21;q21)+-EMZL 患者的 PB 中检测到肿瘤细胞。这些患者的 PB 和胃活检随访样本中肿瘤细胞的存在显示出良好的相关性，表明监测胃 t(11;18)(q21;q21)+-EMZL 患者 PB 中肿瘤细胞的临床相关性。
Gastric extranodal marginal zone lymphoma (EMZL) often shows prolonged localised disease, but the present study demonstrated the presence of tumour cells in peripheral blood (PB) of low stage patients. We studied the presence of tumour cells in PB in gastric lymphoma patients harbouring or lacking t(11;18)(q21;q21), by real‐time immunoglobulin (Ig)H allele‐specific oligonucleotide‐polymerase chain reaction (ASO‐PCR) and API2‐MALT1 PCR. Tumour cells were exclusively detected in PB of t(11;18)(q21;q21)+‐EMZL patients. The presence of tumour cells in PB and gastric biopsy follow‐up samples showed a good correlation in these patients, suggesting clinical relevance for monitoring of tumour cells in PB of gastric t(11;18)(q21;q21)+‐EMZL patients.