Impact of radio frequency electromagnetic radiation on DNA integrity in the male germline
Impact of radio frequency electromagnetic radiation on DNA integrity in the male germline
复制标题
DOI：
10.1111/j.1365-2605.2005.00531.x
复制
发表时间：
2005-06-01
影响因子：
--
通讯作者：
King, BV
中科院分区：
文献类型：
--
作者：
Aitken, RJ;Bennetts, LE;King, BV
人类暴露于射频电磁辐射（PFEMR）引起了关注，包括最近的一份报告表明，经常使用移动电话会对人类精液质量产生负面影响。如果RFEMR的生物学效应包括诱导男性生殖细胞的DNA损伤，那么这些影响将特别严重。在这项研究中，小鼠在波导内以约90 mW/kg的特定吸收率暴露于900 MHz RFEMR，每天12小时，持续7天。暴露后，采用定量PCR （QPCR）以及碱性和脉冲场凝胶电泳评估尾侧附睾精子的DNA损伤。治疗后小鼠明显正常，包括精子数量、形态和活力在内的所有评估标准均未受到显著影响。凝胶电泳显示，没有明显的证据表明，单或双dna链断裂增加的精子从治疗动物。然而，使用QPCR对DNA完整性的详细分析显示，线粒体基因组（p < 0.05）和核β -珠蛋白位点（p < 0.01）的损伤具有统计学意义。本研究表明，虽然RFEMR对男性生殖细胞发育没有显著影响，但对附睾精子有明显的遗传毒性作用，值得进一步研究。
Concern has arisen over human exposures to radio frequency electromagnetic radiation (PFEMR), including a recent report indicating that regular mobile phone use can negatively impact upon human semen quality. These effects would be particularly serious if the biological effects of RFEMR included the induction of DNA damage in male germ cells. In this study, mice were exposed to 900 MHz RFEMR at a specific absorption rate of approximately 90 mW/kg inside a waveguide for 7 days at 12 h per day. Following exposure, DNA damage to caudal epididymal spermatozoa was assessed by quantitative PCR (QPCR) as well as alkaline and pulsed-field gel electrophoresis. The treated mice were overtly normal and all assessment criteria, including sperm number, morphology and vitality were not significantly affected. Gel electrophoresis revealed no gross evidence of increased single- or double-DNA strand breakage in spermatozoa taken from treated animals. However, a detailed analysis of DNA integrity using QPCR revealed statistically significant damage to both the mitochondrial genome (p < 0.05) and the nuclear beta-globin locus (p < 0.01). This study suggests that while RFEMR does not have a dramatic impact on male germ cell development, a significant genotoxic effect on epididymal spermatozoa is evident and deserves further investigation.