A powerful GABA(B) receptor-mediated inhibition of GABAergic neurons in the arcuate nucleus.
A powerful GABA(B) receptor-mediated inhibition of GABAergic neurons in the arcuate nucleus.
复制标题
对弓状核中 GABA 能神经元的强大 GABA(B) 受体介导抑制。
DOI：
10.1097/00001756-199812210-00032
复制
发表时间：
1998
期刊：
影响因子：
1.7
通讯作者：
Rønnekleiv,K
中科院分区：
文献类型：
--
作者：
Wagner,EJ;Bosch,MA;Kelly,MJ;Rønnekleiv,K
WE combined histofluorescence with in sit u hybridization to identify GABAergic neurons in the arcuate nucleus (ARC) following electrophysiological recordings, using GAD 65 as a marker. Intracellular recordings were made in hypothalamic slices prepared from ovariectomized guinea pigs. Over 90% of ARC neurons tested with the GABA B receptor agonist baclofen responded with a membrane hyperpolarization or an outward current. The hyperpolarization was dose-dependent, and the GABA B receptor antagonist CGP 35,348 produced a rightward shift in the agonist dose-response curve. Agonist potency was lower, and the efficacy greater, in GAD-positive neurons. The use of this novel technique for identifying GABAergic neurons thus reveals differences in the pharmacodynamics of GABA B receptor activation GABAergic and non-GABAergic ARC neurons
