A unique type I keratin intermediate filament gene family is abundantly expressed in the inner root sheaths of sheep and human hair follicles
A unique type I keratin intermediate filament gene family is abundantly expressed in the inner root sheaths of sheep and human hair follicles
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DOI：
10.1046/j.1523-1747.2001.00215.x
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发表时间：
2001-01-01
影响因子：
6.5
通讯作者：
Rogers, G
中科院分区：
文献类型：
--
作者：
Bawden, CS;McLaughlan, C;Rogers, G
在绵羊和人的毛囊内毛根鞘中发现了一个独特的I型角蛋白中间丝群，它由三个高度相关的蛋白组成。来自这些物种的第一个成员被命名为oIRSa 1和hIRSa 1，每个编码450个氨基酸的蛋白质，其组成特征介于先前描述的毛发角蛋白和表皮细胞角蛋白I型中间丝之间。检测丰富的mRNA转录来自绵羊和人类基因的cRNA原位杂交只在内根鞘，而不是在髓质同意与早期的超微结构分析的结果，报告中间丝只在内根鞘。IRSa角蛋白基因的聚类在两个物种的基因组中是明显的。这三个hIRSa基因位于人类17号染色体上，与另外三个功能未知的I型角蛋白中间丝基因紧密连锁。（hRPK.142_H_19），可能位于17 q12-q21的I型中间丝细胞角蛋白和毛发角蛋白基因位点附近，包括所有已知的人类I型中间丝细胞角蛋白，hHa角蛋白，hIRSa，和hIRSa连接的角蛋白的系统发育分析表明，起源的IRSa角蛋白中间丝连接组之前的起源的大多数表皮细胞角蛋白和所有的头发角蛋白在出现的角蛋白中间丝基因。
A unique type I keratin intermediate filament group, comprising three highly related proteins and expressed in the inner toot sheath of hair follicles, has been identified in both sheep and human. The first members from these species are named oIRSa1 and hIRSa1 and each encodes a protein of 450 amino acids, with compositional characteristics intermediate between those of previously described hair keratin and epidermal cytokeratin type I intermediate filaments. Detection of abundant mRNA transcripts derived from the sheep and human genes by cRNA in situ hybridization only in the inner root sheath and not in the medulla concurs with the findings of earlier ultrastructural analyses that have reported intermediate filaments only in the inner root sheath. Clustering of the IRSa keratin genes is apparent in the genomes of both species. The three hIRSa genes, known to reside on human chromosome 17, are closely linked to three further type I keratin intermediate filament genes of unknown function, This new gene complex, contained almost entirely within a 156 kb BAC (hRPK.142_H_19), is likely to lie near the type I intermediate filament cytokeratin and hair keratin gene loci at 17q12-q21, A phylogenetic analysis including all known human type I intermediate filament cytokeratins, hHa keratins, hIRSa, and hIRSa-linked keratins suggests that origin of the IRSa keratin intermediate filament linkage group preceded origin of most of the epidermal cytokeratins and all hair keratins during emergence of the keratin intermediate filament genes.