Controlling primary hepatocyte adhesion and spreading on protein-free polyelectrolyte multilayer films
Controlling primary hepatocyte adhesion and spreading on protein-free polyelectrolyte multilayer films
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DOI：
10.1021/ja046188u
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发表时间：
2004-12-22
影响因子：
15
通讯作者：
Chan, C
中科院分区：
文献类型：
--
作者：
Kidambi, S;Lee, I;Chan, C
开发新的方法来制造薄膜，提供精确的控制的三维形貌和细胞粘附可能会导致组织工程和生物传感器领域的重大进展。本文描述了在不使用胶原蛋白或纤连蛋白等粘附蛋白的情况下，原代肝细胞成功地附着和铺展在多层膜（PEM）上。我们证明，附着和扩散的原代肝细胞可以控制使用这种逐层沉积的离子聚合物。在我们的研究中，我们使用合成的聚合物，即聚（二烯丙基二甲基氯化铵）（PDAC）和磺化聚（苯乙烯）（SPS）作为聚阳离子和聚阴离子，分别建立多层膜。原代肝细胞在SPS表面的附着和扩散优先于PDAC表面。SPS图案形成在PEM表面上，通过将SPS微接触印刷到PDAC表面上或反之亦然，以获得原代肝细胞的图案。PEM是一种有用的技术，用于在无蛋白质的环境中制造具有特定细胞-细胞和细胞-表面相互作用的受控共培养物，从而为组织工程应用设计细胞特异性表面提供灵活性。
The development of new methods for fabricating thin films that provide precise control of the three-dimensional topography and cell adhesion could lead to significant advances in the fields of tissue engineering and biosensors. This Communication describes the successful attachment and spreading of primary hepatocytes on polyelectrolyte multilayer (PEM) films without the use of adhesive proteins such as collagen or fibronectin. We demonstrate that the attachment and spreading of primary hepatocytes can be controlled using this layer-by-layer deposition of ionic polymers. In our study, we used synthetic polymers, namely poly(diallyldimethylammonium chloride) (PDAC) and sulfonated poly(styrene) (SPS) as the polycation and polyanion, respectively, to build the multilayers. Primary hepatocytes attached and spread preferentially on SPS surfaces over PDAC surfaces. SPS patterns were formed on PEM surfaces, either by microcontact printing of SPS onto PDAC surfaces or vice versa, to obtain patterns of primary hepatocytes. PEM is a useful technique for fabricating controlled co-cultures with specified cell−cell and cell−surface interactions on a protein-free environment, thus providing flexibility in designing cell-specific surfaces for tissue engineering applications.