Employing ProteoWizard to Convert Raw Mass Spectrometry Data.

Employing ProteoWizard to Convert Raw Mass Spectrometry Data.
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DOI:
10.1002/0471250953.bi1324s46
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发表时间:
2014-06-17
影响因子:
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通讯作者:
Mallick, Parag
Mallick, Parag
中科院分区:
其他
文献类型:
--
作者:
Holman, Jerry D;Tabb, David L;Mallick, Parag

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在生物 LC-MS/MS 实验中,质谱仪捕获原始数据后,必须将其从供应商特定的二进制文件转换为开放格式文件,以便大多数软件进行操作。该协议详细介绍了 ProteoWizard 软件进行此转换的使用,并考虑了格式特征、编码选项和供应商的特殊性。该协议将帮助研究人员准备数据,以便通过数据库搜索引擎和其他生物信息学工具进行分析。
After raw data have been captured by mass spectrometers in biological LC-MS/MS experiments, they must be converted from vendor-specific binary files to open-format files for manipulation by most software. This protocol details the use of ProteoWizard software for this conversion, taking format features, coding options, and vendor particularities into account. This protocol will aid researchers in preparing their data for analysis by database search engines and other bioinformatics tools.