The Proliferation and Differentiation of Neonatal Epidermal Melanocytes in F1 Hairless Mice of HR‐1 × HR/De in Serum‐Free Culture
The Proliferation and Differentiation of Neonatal Epidermal Melanocytes in F1 Hairless Mice of HR‐1 × HR/De in Serum‐Free Culture
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HR-1×HR/De无血清培养F1代无毛小鼠新生表皮黑素细胞的增殖和分化
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
T. Hirobe
中科院分区：
文献类型：
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作者：
R. Furuya;S. Akiu;M. Naganuma;M. Fukuda;T. Hirobe
为研究HR-1 × HR/De亲代F1代无毛小鼠表皮黑素细胞的体外增殖和分化特性，将新生表皮细胞悬液培养于添加有二丁酰腺苷3 '，5'-环磷酸（DBcAMP）和/或碱性成纤维细胞生长因子（bFGF）的无血清培养基中。用DBcAMP处理黑素细胞诱导黑素细胞分化。相反，DBcAMP和bFGF联合处理可诱导成黑素细胞持续增殖。在存在角质形成细胞的情况下，成黑素细胞可以在补充有两种因子的无血清培养基中传代培养，但在不存在角质形成细胞的情况下则不行。这是首次成功培养无毛小鼠的成黑素细胞和黑素细胞的报告;预计这将有助于理解（HR-1 × HR/De）F1小鼠表皮色素斑的形成机制，据报道，该色素斑是由反复暴露于紫外线B诱导的。
To investigate the characteristics of the proliferation and differentiation of epidermal melanocytes in F1 hairless mice of HR‐1 × HR/De parents in vitro, cell suspensions of the neonatal epidermis were cultured in a serum‐free medium supplemented with dibutyryl adenosine 3‘,5‘‐cyclic monophosphate (DBcAMP) and/or basic fibroblast growth factor (bFGF). The differentiation of melanocytes was induced by treatment with DBcAMP. In contrast, the sustained proliferation of melanoblasts was induced by combined treatment with DBcAMP and bFGF. The melanoblasts could be subcultured in serum‐free medium supplemented with the two factors in the presence of keratinocytes, but not in their absence. This is the first report of successful culture of melanoblasts and melanocytes from hairless mice; it is expected to be useful in understanding the mechanism of the development of pigmented spots in the epidermis of (HR‐1 × HR/De) F1 mice, which are reported to be induced by repeated exposure to ultraviolet light B.
影响因子：
6.5
作者：
GORDON, PR;MANSUR, CP;GILCHREST, BA
通讯作者：
GILCHREST, BA
未分化的角质形成细胞通过细胞间接触控制正常黑素细胞的生长、形态和抗原表达。
DOI：
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发表时间：
1993
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
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作者：
Valyi-Nagy,IT;Hirka,G;Jensen,PJ;Shih,IM;Juhasz,I;Herlyn,M
通讯作者：
Herlyn,M