Cooperation between giant DNA molecules and actin filaments in a microsphere
Cooperation between giant DNA molecules and actin filaments in a microsphere
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DOI：
10.1103/physreve.81.051921
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发表时间：
2010-05-01
影响因子：
2.4
通讯作者：
Yoshikawa, Kenichi
中科院分区：
文献类型：
--
作者：
Negishi, Makiko;Sakaue, Takahiro;Yoshikawa, Kenichi
我们报告了在微球中由于肌动蛋白丝和巨大DNA分子之间的合作而出现的空间分离状态。当共存的肌动蛋白浓度足够高时，DNA分子通过与肌动蛋白丝形成一个组装体而被排除在表面。随着肌动蛋白浓度的降低，肌动蛋白丝倾向于溶解在球内，而DNA分子仍然被排除在表面。当肌动蛋白浓度变得更低时，DNA分子通过避免表面附着而溶解在球体内。我们通过引入微球内肌动蛋白丝的“禁区”概念来解释这些实验趋势。
We report the appearance of a spatially segregated state in a microsphere as a result of cooperation between actin filaments and giant DNA molecules. When the coexisting actin concentration is high enough, DNA molecules are excluded toward the surface by forming an assembly with actin filaments. With a decrease in the actin concentration, actin filaments tend to dissolve within the sphere whereas DNA molecules remain to be excluded onto the surface. When the actin concentration becomes still lower, DNA molecules dissolve within the sphere by avoiding surface attachment. We interpret these experimental trends by introducing the concept of an "exclusion zone" for actin filaments within a microsphere.