Liquid chromatography/mass spectrometry analysis of mixtures of rhamnolipids produced by Pseudomonas aeruginosa strain 57RP grown on mannitol or naphthalene
Liquid chromatography/mass spectrometry analysis of mixtures of rhamnolipids produced by Pseudomonas aeruginosa strain 57RP grown on mannitol or naphthalene
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DOI：
10.1016/s1388-1981(99)00129-8
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发表时间：
1999-09-22
影响因子：
4.8
通讯作者：
Villemur, R
中科院分区：
文献类型：
--
作者：
Déziel, E;Lépine, F;Villemur, R
采用电喷雾电离液相色谱/质谱法分析了以甘露醇或萘为碳源的铜绿假单胞菌产鼠李糖脂。使用反相C-18色谱柱和30 min的色谱运行完成了28种不同鼠李糖脂同源物的鉴定和定量。通过质谱的解释和相对比例的估算，可以对未被色谱分离的异构鼠李糖脂进行鉴别。甘露醇中最丰富的鼠李糖脂含有两个鼠李糖和两个3-羟基癸酸基团。萘中含量最多的鼠李糖脂含有两个鼠李糖和一个3-羟基癸酸基团。(C) 1999 Elsevier Science B.V.版权所有
Liquid chromatography/mass spectrometry using electrospray ionisation was used to analyse rhamnolipids produced by a Pseudodomonas aeruginosa strain with mannitol or naphthalene as carbon source. Identification and quantification of 28 different rhamnolipid congeners was accomplished using a reverse-phase C-18 column and a 30 min chromatographic run. Isomeric rhamnolipids that were not chromatographically resolved could be identified by interpretation of their mass spectra and their relative proportions estimated. The most abundant rhamnolipid produced on mannitol contained two rhamnoses and two 3-hydroxydecanoic acid groups. The most abundant rhamnolipid produced from naphthalene contained two rhamnoses and one 3-hydroxydecanoic acid group. (C) 1999 Elsevier Science B.V. All rights reserved.