Seasonal changes in the spermatogenesis of the large Japanese field mice (Apodemus speciosus) controlled by proliferation and apoptosis of germ cells
Seasonal changes in the spermatogenesis of the large Japanese field mice (Apodemus speciosus) controlled by proliferation and apoptosis of germ cells
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DOI：
10.1016/j.anireprosci.2020.106288
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发表时间：
2020-03-01
影响因子：
2.2
通讯作者：
Yamashiro, Hideaki
中科院分区：
文献类型：
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作者：
Ito, Jun;Meguro, Kanna;Yamashiro, Hideaki
本研究的目的是探讨季节性生殖周期中的雄性生殖细胞的增殖和凋亡的日本大田鼠（Apodemus speciosus）。在日本新泻捕获了居住在其自然栖息地的雄性小鼠。睾丸切片用苏木精和伊红染色，有丝分裂的雄性生殖细胞用增殖细胞核抗原（PCNA）免疫荧光染色鉴定。采用末端脱氧核苷酸转移酶（TdT）介导的脱氧尿苷三磷酸（dUTP）缺口末端标记（TUNEL）法分析细胞凋亡。根据生精小管的直径，将季节性生殖周期中精子发生的阶段分为活跃期、过渡期和非活跃期。非活动期PCNA阳性生殖细胞数少于其他各期。TUNEL阳性生殖细胞的百分比，每个曲细精管是更大的非活动期比活动期和过渡期。季节性生殖周期中的精子发生受雄性生殖细胞的增殖和凋亡控制。这种未驯化的小鼠可用作研究精子发生的动物模型，作为生态和人为因素对动物生殖影响的有价值的指标。
The aim of this study was to investigate the proliferation and apoptosis of male germ cells during the seasonal reproductive cycle of the large Japanese field mice (Apodemus speciosus). Male mice residing in their natural habitat were captured in Niigata, Japan. Testis sections were stained with haematoxylin and eosin, and mitotic male germ cells were identified using immunofluorescence staining for proliferating cell nuclear antigen (PCNA). Apoptosis was analysed using terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) nick end labelling (TUNEL) assay. The phases of spermatogenesis during the seasonal reproductive cycle were classified as active, transitional, and inactive based on the diameter of the seminiferous tubules. The number of PCNA-positive germ cells was less during the inactive than other phases. The percentage of TUNEL-positive germ cells per seminiferous tubule was greater during the inactive than active and transitional phases. Spermatogenesis during the seasonal reproductive cycle is controlled by proliferation and apoptosis in male germ cells. This species of undomesticated mice could be used as an animal model to study spermatogenesis as a valuable indicator of the effects of ecological and anthropogenic factors on animal reproduction.