Isolation of respiratory bovine coronavirus, other cytocidal viruses, and Pasteurella spp from cattle involved in two natural outbreaks of shipping fever
Isolation of respiratory bovine coronavirus, other cytocidal viruses, and Pasteurella spp from cattle involved in two natural outbreaks of shipping fever
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DOI：
10.2460/javma.2000.216.1599
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发表时间：
2000-05-15
期刊：
影响因子：
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通讯作者：
Loan, RW
中科院分区：
文献类型：
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作者：
Storz, J;Purdy, CW;Loan, RW
1997年和1998年爆发的两次运输热自然暴发中，分别采集了105头和120头阉割的4- 8月龄雄性饲养场牛，以鉴定可从牛中分离的杀细胞病毒和巴斯德氏菌属。牛在到达来自4家当地拍卖行的订单买家谷仓时接种疫苗。四天后，他们被运送到饲养场，并收集了额外的鼻拭子标本和血液样本。送检鼻拭子标本进行病毒分离和细菌培养;结果1997年和1998年期间，105头牛中有93头和120头牛中有106头出现呼吸道疾病症状，分别从81头和89头病牛中分离出RBCV，分别在订单购买者的谷仓或到达饲养场后的第二天。在1997年爆发期间，从订购者畜棚的2头牛和到达饲养场后7天和14天的5头牛中分离出牛疱疹病毒1，从到达饲养场后14天的4头牛中分离出副流感病毒3。在1998年爆发期间，从订购者畜棚和到达饲养场的2头牛以及到达饲养场后7天和14天的5头牛中分离出牛疱疹病毒1型，从到达饲养场当天的1头动物以及7天和14天后的18头牛中分离出副流感病毒3型。巴氏杆菌属培养4和6牛在订单买方的谷仓和92和72牛在1997年和1998年的爆发，分别抵达饲养场，结论和临床相关性结果表明，RBCV可能发挥致病作用，在牛的航运热爆发。在2次暴发初期，当巴氏杆菌感染率较低时，80%以上的病牛排出RBCV。
Objective-To identify cytocidal viruses and Pasteurella spp that could be isolated from cattle involved in 2 natural outbreaks of shipping fever.Animals-105 and 120 castrated male 4- to 8-month-old feedlot cattle involved in 1997 and 1998 outbreaks, respectively.Procedures-Nasal swab specimens and blood samples were collected, and cattle were vaccinated on arrival at an order-buyer barn from 4 local auction houses. Four days later, they were transported to a feedlot, and additional nasal swab specimens and blood samples were collected. Nasal swab specimens were submitted for virus isolation and bacterial culture; blood samples were submitted for measurement of respiratory bovine coronavirus (RBCV) hemagglutinin inhibition titers.Results-93 of 105 cattle and 106 of 120 cattle developed signs of respiratory tract disease during 1997 and 1998, respectively, and RBCV was isolated from 81 and 89 sick cattle, respectively while at the order-buyer's barn or the day after arrival at the feedlot. During the 1997 outbreak, bovine herpesvirus 1 was isolated from 2 cattle at the order-buyer's barn and from 5 cattle 7 and 14 days after arrival at the feedlot, and parainfluenza virus 3 was isolated from 4 cattle 14 days after arrival at the feedlot. During the 1998 outbreak, bovine herpesvirus 1 was isolated from 2 cattle at the order-buyer's barn and on arrival at the feedlot and from 5 cattle 7 and 14 days after arrival at the feedlot, and parainfluenza virus 3 was isolated from 1 animal the day of, and from 18 cattle 7 and 14 days after, arrival at the feedlot. Pasteurella spp was cultured from 4 and 6 cattle at the order-buyer's barn and from 92 and 72 cattle on arrival at the feedlot during the 1997 and 1998 outbreaks, respectively.Conclusions and Clinical Relevance-Results suggest that RBCV may play a causative role in outbreaks of shipping fever in cattle. More than 80% of the sick cattle shed RBCV at the beginning of 2 outbreaks when the Pasteurella spp infection rate was low.