RAS (PROTO)ONCOGENE INDUCES N-LINKED CARBOHYDRATE MODIFICATION - TEMPORAL RELATIONSHIP WITH INDUCTION OF INVASIVE POTENTIAL
RAS (PROTO)ONCOGENE INDUCES N-LINKED CARBOHYDRATE MODIFICATION - TEMPORAL RELATIONSHIP WITH INDUCTION OF INVASIVE POTENTIAL
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DOI：
10.1002/j.1460-2075.1988.tb03208.x
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发表时间：
1988-11-01
期刊：
影响因子：
11.4
通讯作者：
PLOEGH, HL
中科院分区：
文献类型：
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作者：
BOLSCHER, JGM;VANDERBIJL, MMW;PLOEGH, HL
研究ras癌基因表达对蛋白糖基化的影响。分析VSV G蛋白和I类组织相容性抗原，以监测ras介导的糖基化变化。瞬时表达的c-Ha-ras癌基因，引入NIH 3 T3细胞的DEAE-葡聚糖的方法，改变蛋白质糖基化在25小时内转染。地塞米松诱导p21-ras在稳定的NIH 3 T3转染子中表达后也得到了相同的结果，该转染子含有在糖皮质激素诱导的MMTV启动子控制下的激活的Ha-ras癌基因或正常的N-ras原癌基因。细胞表面碳水化合物的改变，诱导ras（原）癌基因和随后收购的侵入性的潜力，发生在形态转化。
The effect of expression of the ras oncogene on protein glycosylation was studied. VSV G-protein and class I histocompatibility antigens were analysed to monitor ras-mediated changes in glycosylation. Transient expression of the c-Ha-ras oncogene, introduced into NIH 3T3 cells by the DEAE-dextran method, altered protein glycosylation within 25 h of transfection. The same result was obtained after dexamethasone-induced expression of p21-ras in stable NIH 3T3 transfectants containing either an activated Ha-ras oncogene or a normal N-ras protooncogene under control of the glucocorticoid-inducible MMTV promoter. The alteration of cell surface carbohydrates, induced by the ras (proto)oncogene and the subsequent acquisition of invasive potential, occurred prior to morphological transformation.