Cytogenetic characterization of the Arabidopsis thaliana natural tetraploid ecotype Warschau stability during in vitro regeneration
Cytogenetic characterization of the Arabidopsis thaliana natural tetraploid ecotype Warschau stability during in vitro regeneration
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DOI：
10.1007/s11240-016-1006-5
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发表时间：
2016-05
期刊：
影响因子：
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通讯作者：
Maja Orzechowska;Sylwia Gurdek;D. Siwińska;Anna Piekarska-Stachowiak
中科院分区：
文献类型：
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作者：
Maja Orzechowska;Sylwia Gurdek;D. Siwińska;Anna Piekarska-Stachowiak
研究了天然同源四倍体拟南芥(Wa-1)的形态和细胞遗传学特征。与二倍体哥伦比亚植物相比，所分析的大多数华夏植物器官的大小都较高。通过有丝分裂中期细胞分析确定了四倍体染色体数目，并对rDNA进行了定位。35S rDNA基因座位于第2、4号染色体上，5S rDNA基因座位于A。同源四倍体材料用于体外培养，研究体细胞无性系变异。通过器官发生实现了高效的再生。大多数试管苗的倍性水平保持不变。对再生植株进行了详细的细胞遗传学分析，包括染色体、染色中心和rDNA信号数，显示再生植株的稳定性。基于这些数据，我们建议将生态型Warschau作为一种具有良好特性的植物材料，用于未来研究多倍体对基因组的影响。
The morphological and cytogenetic features of the natural autotetraploidArabidopsis thalianaecotype Warschau (Wa-1) were investigated. Most of the Warschau plant organs that were analyzed showed higher size values in comparison with diploid Columbia plants. The tetraploid chromosome number was confirmed by analysis of mitotic metaphase cells and rDNA loci were localized. 35S rDNA loci were present on chromosomes 2 and 4, while 5S rDNA, which is polymorphic amongA. thalianaecotypes, were present on chromosomes 4 and 5. Well-characterized autotetraploid plant material was used for in vitro culture to investigate somaclonal variation. Efficient regeneration through organogenesis was achieved. Most of the plants obtained in vitro exhibited an unchanged ploidy level. Detailed cytogenetic analysis that included chromosome, chromocenters and rDNA signals numbers, revealed the stability of regenerants. Based on these data we recommend the ecotype Warschau as a well-characterized plant material for future investigations on the consequences of polyploidy for the genome.