Role of the Vps9-domain protein RgfA in Dictyostelium chemotaxis and development.
Role of the Vps9-domain protein RgfA in Dictyostelium chemotaxis and development.
复制标题
Vps9 结构域蛋白 RgfA 在盘基网柄菌趋化和发育中的作用。
DOI：
10.1139/cjm-2012-0519
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发表时间：
2013
影响因子：
2.8
通讯作者：
Hadwiger,JeffreyA
中科院分区：
文献类型：
--
作者：
Hadwiger,JeffreyA
具有Vps9结构域的蛋白质作为Rab蛋白的鸟嘌呤核苷酸交换因子起作用，并且可以通过胞吞作用介导细胞表面受体或其它分子的摄取。然而，编码这些蛋白质的基因以前没有在具有强大趋化能力的细胞中进行过研究。在盘基网柄藻的基因组中鉴定了几个编码Vps9结构域的基因，并检测了其中一个基因rgfA（DDB_G0272038）在细胞生长、发育和趋化性中的功能。ThergfA基因在营养生长和整个发育过程中表达，但该基因的破坏导致细胞生长，巨胞饮，发育形态或趋化运动没有重大改变。然而，异源表达的RgfA导致发育形态发生延迟和受损的细胞对叶酸的趋化性，但不影响巨胞饮。这些结果表明，RgfA可能与其他网柄菌Vps9结构域蛋白共享冗余功能，并且该蛋白的异源表达可以改变依赖于外部信号接收的过程。
Proteins with a Vps9 domain function as guanine nucleotide exchange factors for Rab proteins and can mediate the uptake of cell surface receptors or other molecules through endocytosis. However, genes encoding these proteins have not been previously studied in cells with robust chemotactic capabilities. Several genes encoding Vps9 domains were identified in the genome ofDictyostelium discoideum, and one of the genes, designated asrgfA(DDB_G0272038), was examined for functions in cell growth, development, and chemotaxis. ThergfAgene was expressed during vegetative growth and throughout development, but disruption of this gene resulted in no major alterations in cell growth, macropinocytosis, developmental morphology, or chemotactic movement. However, heterologous expression of RgfA resulted in a delay of developmental morphogenesis and impaired chemotaxis of cells to folate but did not affect macropinocytosis. These results suggest that RgfA might share redundant functions with otherDictyosteliumVps9-domain proteins and that heterologous expression of this protein can alter processes that depend on the reception of external signals.