Bdellovibrio bacteriovorus HD100, a predator of Gram-negative bacteria, benefits energetically from Staphylococcus aureus biofilms without predation
Bdellovibrio bacteriovorus HD100, a predator of Gram-negative bacteria, benefits energetically from Staphylococcus aureus biofilms without predation
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DOI：
10.1038/s41396-018-0154-5
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发表时间：
2018-08-01
期刊：
影响因子：
11
通讯作者：
Mitchell, Robert J.
中科院分区：
文献类型：
--
作者：
Im, Hansol;Dwidar, Mohammed;Mitchell, Robert J.
蜈蚣弧菌HD100是一种捕食性细菌，它通过入侵革兰氏阴性细菌的周质并从内部消耗它们而生存。虽然杆菌HD100只攻击革兰氏阴性细菌菌株，但我们的工作表明，攻击期的捕食细胞也受益于使用金黄色葡萄球菌生物膜与革兰氏阳性生物膜的相互作用，我们表明这种捕食者降解生物膜基质，获得营养物质，并利用这些来生产和分泌蛋白水解酶来继续这一过程。当暴露在金黄色葡萄球菌生物膜中时，细小杆菌HD100的转录组与在胞质内时看到的相似，这表明它的反应与在猎物中相似。此外，对其中两种诱导酶(Bd2269和Bd2692)进行了纯化，并验证了它们对金黄色葡萄球菌生物被膜的抑制活性。此外，细菌杆菌HD100从与金黄色葡萄球菌生物膜的相互作用中获得了几个明显的好处，包括增加了ATP池，并在提供猎物时改善了下游的捕食活动。
Bdellovibrio bacteriovorus HD100 is a predatory bacterium which lives by invading the periplasm of Gram-negative bacteria and consuming them from within. Although B. bacteriovorus HD100 attacks only Gram-negative bacterial strains, our work here shows attack-phase predatory cells also benefit from interacting with Gram-positive biofilms Using Staphylococcus aureus biofilms, we show this predator degrades the biofilm matrix, obtains nutrients and uses these to produce and secrete proteolytic enzymes to continue this process. When exposed to S. aureus biofilms, the transcriptome of B. bacteriovorus HD100 was analogous to that seen when present intraperiplasmically, suggesting it is responding similarly as when in a prey. Moreover, two of the induced proteases (Bd2269 and Bd2692) were purified and their activities against S. aureus biofilms verified. In addition, B. bacteriovorus HD100 gained several clear benefits from its interactions with S. aureus biofilms, including increased ATP pools and improved downstream predatory activities when provided prey.